Beilong virus, a novel paramyxovirus with the largest genome of non-segmented negative-stranded RNA viruses
Beilong virus, a novel paramyxovirus with the largest genome of non-segmented negative-stranded RNA viruses
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DOI：
10.1016/j.virol.2005.10.039
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发表时间：
2006-03-01
期刊：
影响因子：
3.7
通讯作者：
Wang, LF
中科院分区：
文献类型：
--
作者：
Li, Z;Yu, M;Wang, LF
在对人肾系膜细胞（HMCs）基因表达的减法研究中，分离到了与副粘病毒P、M和F基因序列同源的cDNA克隆。随后的调查显示，这种特殊的HMC系感染了一种以前未知的副粘病毒。在这里，我们报道了这种新病毒的分离和基因组特征，现在被命名为北龙病毒（BeV）。BeV的基因组长度为19,12个核苷酸（nt），是所有已知的单胞病毒目成员中最大的。BeV基因组包含8个基因，序列为3′-N-P/V/C-M-F-SH-TM-G-L-5′。SH和TM基因分别编码一个76氨基酸的疏水小蛋白和一个254氨基酸的跨膜蛋白。BeV G基因位于4527 nt，编码734 aa的附着蛋白，在基因的3'半部分包含两个额外的开放阅读框（OPFs），编码299和394 an的推定蛋白。虽然BeV的确切起源目前尚不清楚，但我们提供的证据表明，在获得HMC细胞系之前，在同一实验室使用的大鼠系膜细胞系中存在BeV，这表明BeV可能起源于啮齿动物。(c) 2005爱思唯尔公司版权所有。
During a subtraction study on gene expression in human kidney mesangial cells (HMCs), cDNA clones with sequence homology to paramyxovirus P, M and F genes were isolated. Subsequent investigation revealed that this particular HMC line was infected with a previously Unknown paramyxovirus. Here, we report the isolation and genome characterization of this new virus, now named Beilong virus (BeV). The genome of BeV is 19,2 12 nucleotides (nt) in length and is the largest among all known members of the order Mononegavirales. The BeV genome contains eight genes in the order 3'-N-P/V/C-M-F-SH-TM-G-L-5'. The SH and TM genes code for a small hydrophobic protein of 76 an and a transmembrane protein of 254 aa, respectively. The BeV G gene, at 4527 nt, codes for an attachment protein of 734 aa and contains two additional open reading frames (OPFs) in the 3' half of the gene, coding for putative proteins of 299 and 394 an in length. Although the exact origin of BeV is presently unknown, we provide evidence indicating that BeV was present in a rat mesangial cell line used in the same laboratory prior to the acquisition of the HMC line, suggesting a potential rodent origin for BeV. (c) 2005 Elsevier Inc. All rights reserved.