A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae
A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae
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DOI：
10.1126/science.1065672
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发表时间：
2001-12-21
期刊：
影响因子：
56.9
通讯作者：
Boeke, JD
中科院分区：
文献类型：
--
作者：
Ooi, SL;Shoemaker, DD;Boeke, JD
我们描述了一种基于微阵列的筛选，通过对数千个酵母突变体进行不同的遗传选择来进行，这些突变体代表了酵母基因组中的大多数基因。突变体的存在或不存在通过与20个核苷酸“条形码”杂交的寡核苷酸阵列来检测。“我们使用这种方法来筛选非同源末端连接（NHEJ）途径的组分。鉴定了该途径的已知组分，以及先前不知道参与NHEJ的基因NEJ 1。Nej 1蛋白与LIF 1/XRCC 4的氨基末端相互作用，LIF 1/XRCC 4是最近公认的抗癌“基因组守护者”。
We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.