Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance
Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance
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DOI：
10.1038/83505
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发表时间：
2001-01-01
影响因子：
46.9
通讯作者：
Saito, I
中科院分区：
文献类型：
--
作者：
Nakatani, K;Sando, S;Saito, I
在这里，我们设计并合成了配体，其以高亲和力（Kd = 53 nM）特异性结合鸟嘌呤（G）-鸟嘌呤错配，这是四种单核苷酸多态性（SNP）之一。G-G错配的检测通过表面等离子体共振（SPR）测定使用在其表面上携带G-G特异性配体的传感器芯片来进行。通过仅对含有G-G错配的DNA获得显著的SPR响应，证明了SPR传感器检测G-G错配的准确性。含有G-A和G-T错配的DNA，以及完全匹配的双链体，只产生微弱的反应。此外，发现该测定适用于检测HSP 70 -2基因的652个核苷酸序列的PCR扩增产物中存在的SNP。
Here we have designed and synthesized ligands that specifically bind with high affinity (K-d = 53 nM) to the guanine (G)-guanine mismatch, one of four types of single-nucleotide polymorphism (SNP). Detection of the G-G mismatch was performed by a surface plasmon resonance (SPR) assay using a sensor chip carrying the G-G specific ligand on its surface. The accuracy of the G-G mismatch detection by the SPR sensor was demonstrated by a marked SPR response obtained only for the DNA containing the G-G mismatch. DNAs containing G-A and G-T mismatches, as well as a fully matched duplex, produced only a weak response. Furthermore, this assay was found applicable for the detection of SNP existing in PCR amplification products of a 652-nucleotide sequence of the HSP70-2 gene.