Comprehensive analysis of the flowering genes in Chinese cabbage and examination of evolutionary pattern of CO-like genes in plant kingdom.
Comprehensive analysis of the flowering genes in Chinese cabbage and examination of evolutionary pattern of CO-like genes in plant kingdom.
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大白菜开花基因综合分析及植物界CO类基因进化模式考察
DOI：
10.1038/srep14631
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发表时间：
2015-09-29
影响因子：
4.6
通讯作者：
Hou X
中科院分区：
文献类型：
--
作者：
Song X;Duan W;Huang Z;Liu G;Wu P;Liu T;Li Y;Hou X
在植物中，开花是从营养生长到生殖生长最重要的过渡。单子叶植物和真双子叶植物的开花模式明显不同，但很少有研究描述它们开花基因的进化模式。在这项研究中，我们分析了这些基因的进化模式、重复和表达水平。主要结果如下：（i）单子叶植物和真双子叶植物开花基因的表征，包括科特异性、直系同源和共线基因的鉴定； (ii) 芸苔中关键开花基因 CONSTANS 样基因（BraCOLgenes）的全面表征； (iii) 植物界COL基因进化探索及COL基因进化模式构建； (iv) 对十字花科和草类的 CO 和 FT 基因进行比较分析，鉴定了几个科特有的氨基酸，并揭示了 B 中的 CO 和 FT 蛋白质结构相似。油菜和拟南芥但在水稻上有所不同； (v)B中光周期途径相关基因的表达分析。通过 RT-qPCR 检测雷柏在不同光周期处理下的情况。该分析将为理解 COL 基因的开花机制和进化模式提供资源。此外，这种COL基因的全基因组比较研究也可能为其他开花基因的进化提供线索。
In plants, flowering is the most important transition from vegetative to reproductive growth. The flowering patterns of monocots and eudicots are distinctly different, but few studies have described the evolutionary patterns of the flowering genes in them. In this study, we analysed the evolutionary pattern, duplication and expression level of these genes. The main results were as follows: (i) characterization of flowering genes in monocots and eudicots, including the identification of family-specific, orthologous and collinear genes; (ii) full characterization of CONSTANS-like genes inBrassica rapa(BraCOLgenes), the key flowering genes; (iii) exploration of the evolution ofCOLgenes in plant kingdom and construction of the evolutionary pattern ofCOLgenes; (iv) comparative analysis ofCOandFTgenes betweenBrassicaceaeandGrass, which identified several family-specific amino acids and revealed that CO and FT protein structures were similar inB. rapaandArabidopsisbut different in rice; and (v) expression analysis of photoperiod pathway-related genes inB. rapaunder different photoperiod treatments by RT-qPCR. This analysis will provide resources for understanding the flowering mechanisms and evolutionary pattern ofCOLgenes. In addition, this genome-wide comparative study ofCOLgenes may also provide clues for evolution of other flowering genes.