One-step method for quantitative and qualitative analysis of fatty acids in marine animal samples
One-step method for quantitative and qualitative analysis of fatty acids in marine animal samples
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DOI：
10.1016/j.jembe.2007.08.024
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发表时间：
2008-01-04
影响因子：
2
通讯作者：
Tsuchiya, Makoto
中科院分区：
文献类型：
--
作者：
Abdulkadir, Sakdullah;Tsuchiya, Makoto
在一步法中，脂肪酸甲酯（FAME）已制备没有事先的脂质提取。每个牡蛎，蛤，鱿鱼样品酯化与14%的三氟化硼（BF3）在甲醇中在100摄氏度120分钟，和己烷被用来帮助溶解脂质。与常规方法相比，该方法得到了更高的脂肪酸回收率。使用内标，可以定量和定性测定样品的脂肪酸含量。该方法具有简便、快速、减少有机溶剂用量等优点，适用于海洋生物样品中脂肪酸组成的测定。(C)2007 Elsevier B.V.保留所有权利。
In the one-step method, fatty acid methyl esters (FAMEs) have been prepared without prior lipid extraction. Each oyster, clam, and squid sample was esterified with 14% boron-trifluoride (BF3) in methanol at 100 degrees C for 120 min, and hexane was used to help dissolve the lipids. This method gave a higher recovery of fatty acids as compared to the conventional method. Using an internal standard, the fatty acid content of the sample can be determined quantitatively and qualitatively. Due to its simplicity, speed, and reduced organic solvent usage, the one-step method should be useful for the determination of fatty acid composition in marine biological samples. (C) 2007 Elsevier B.V. All rights reserved.