Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction
Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction
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DOI：
10.1007/s10265-005-0249-8
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Ito, M
中科院分区：
文献类型：
--
作者：
Hamada, S;Sekimoto, H;Ito, M
肌凝蛋白包括一个大的分子马达超家族，在atp依赖的与肌动蛋白丝的相互作用中产生机械力。根据其保守的运动结构域序列，肌凝蛋白可分为至少17类，其中3类（VIII， XI， XIII）存在于植物中。虽然从几种绿色植物中可以获得肌凝蛋白的完整序列，但对这些蛋白质的功能知之甚少。此外，藻类肌凝蛋白的序列信息不完整，对绿色植物肌凝蛋白的分子进化研究较少。本研究以Closterium peracerosum-strigosum-littorale复合体为模型系统，研究了一种单细胞基础藻。这种生物在两种交配类型之间的有性繁殖方面已经得到了很好的研究。利用设计用于扩增运动结构域序列的简并引物获得了3种属于XI类肌球蛋白的部分序列。实时聚合酶链反应分析显示，其中一个基因在有性繁殖过程中表达量更高，并且在无性生长的细胞中表达依赖于细胞周期。
Myosins comprise a large superfamily of molecular motors that generate mechanical force in ATP-dependent interactions with actin filaments. On the basis of their conserved motor domain sequences, myosins can be divided into at least 17 classes, 3 of which (VIII, XI, XIII) are found in plants. Although full sequences of myosins are available from several species of green plants, little is known about the functions of these proteins. Additionally, sequence information for algal myosin is incomplete, and little attention has been given to the molecular evolution of myosin from green plants. In the present study the Closterium peracerosum-strigosum-littorale complex was used as a model system for investigating a unicellular basal charophycean alga. This organism has been well studied with respect to sexual reproduction between its two mating types. Three types of partial sequences belonging, to class XI myosins were obtained using degenerate primers designed to amplify motor domain sequences. Real-time polymerase chain reaction analysis of the respective myosin genes during various stages of the algal life cycle showed that one of the genes was more highly expressed during sexual reproduction, and that expression was cell-cycle-dependent in vegetatively grown cells.