The primary structure of rat ribosomal protein L14.
The primary structure of rat ribosomal protein L14.
复制标题
大鼠核糖体蛋白L14的一级结构。
DOI：
10.1006/bbrc.1996.0761
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Wool,IG
中科院分区：
文献类型：
--
作者：
Chan,YL;Olvera,J;Wool,IG
从两个重组cDNA中的核苷酸序列推导出大鼠60 S核糖体亚基蛋白L14的氨基酸序列。核糖体蛋白L14有213个氨基酸（mRNA翻译后去除了NH 2-末端的甲硫氨酸）;分子量为23，193。cDNA与核DNA的杂交表明，有6至8个拷贝的L14基因。该蛋白的mRNA长度约为800个核苷酸。大鼠L14与许多以前未鉴定的来自其他真核生物的核糖体蛋白有关，但与古细菌或真细菌的任何蛋白无关。大鼠L14和鸡组蛋白H1的羧基末端氨基酸序列是相关的。黑腹果蝇大鼠L14同源基因突变导致严重的微小表型。
The amino acid sequence of the rat 60S ribosomal subunit protein L14 was deduced from the sequence of nucleotides in two recombinant cDNAs. Ribosomal protein L14 has 213 amino acids (the NH2-terminal methionine is removed after translation of the mRNA); the molecular weight is 23,193. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 6 to 8 copies of the L14 gene. The mRNA for the protein is about 800 nucleotides in length. Rat L14 is related to a number of previously unidentified ribosomal proteins from other eukaryotes but not to any from archaebacteria or eubacteria. The carboxyl-terminal amino acid sequences of rat L14 and of chicken histone H1 are related. Mutation of theDrosophila melanogasterhomolog of rat L14 causes a severeMinutephenotype.