Death associated protein 1 (DAP 1) positively regulates virus replication and apoptosis of hemocytes in shrimp Marsupenaeus japonicus
Death associated protein 1 (DAP 1) positively regulates virus replication and apoptosis of hemocytes in shrimp Marsupenaeus japonicus
复制标题
死亡相关蛋白1（DAP 1）正向调节日本对虾病毒复制和血细胞凋亡
DOI：
10.1016/j.fsi.2017.02.014
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发表时间：
2017-04-01
影响因子：
4.7
通讯作者：
Kang, Cui-Jie
中科院分区：
文献类型：
--
作者：
Xia, Wen-Li;Kang, Li-Hua;Kang, Cui-Jie
死亡相关蛋白1（Death-associated protein 1，DAP 1）是一种富含脯氨酸的小分子胞质蛋白，在哺乳动物的细胞凋亡和自噬过程以及人类的临床肿瘤中发挥重要作用。然而，关于DAP 1的同源基因及其在无脊椎动物生理过程中的作用，目前还知之甚少。本文报道了DAP 1在对虾抗病毒免疫中的新功能。从日本囊对虾（Marsupenaeus）中克隆了DAP 1的同源基因，命名为Mjdap-1。Mjdap-1基因全长1761 bp，开放阅读框309 bp，编码102个氨基酸。逆转录PCR结果表明，Mjdap-1在血细胞、鳃、肠、胃、心脏、肝胰腺、睾丸和卵巢等组织中均有表达。在对虾中，Mjdap-1的转录被白色斑点综合征病毒（WSSV）感染上调，Mjdap-1的敲低降低了病毒在体内的复制和M. WSSV挑战相反，注射纯化的重组MjDAP 1蛋白促进了病毒在虾体内的扩增。流式细胞仪检测结果显示，MjDAP 1蛋白注射可增强病毒感染诱导的血细胞凋亡，而MjDAP 1基因敲除可抑制病毒感染诱导的血细胞凋亡。此外，Mjdap-1可调控凋亡诱导因子（AIF）的表达，但不影响caspase的转录。我们的研究结果表明，MjDAP 1促进病毒在虾体内的扩增，这可能是由于AIF依赖的方式促进血细胞凋亡。这些结果为该蛋白的功能提供了新的见解，可用于病毒病的控制。(C)2017爱思唯尔有限公司版权所有
Death-associated protein 1 (DAP1) is a small praline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. However, little knowledge is known about the homologue gene of DAP1 and its roles in the physiological process of invertebrates. In this paper, we report a novel function of DAP1 in the antivirus immunity of shrimp. A homologue gene of DAP1 was cloned from Marsupenaeus japonicus and named as Mjdap-1. The fulllength of Mjdap-1 was 1761 bp with a 309 bp open reading frame that encoded 102 amino acids. Reverse transcription-PCR results showed that Mjdap-1 was expressed in all tested tissues, including hemocytes, gills, intestines, stomach, heart, hepatopancreas, testes, and ovaries. In shrimp, Mjdap-1 transcripts were up-regulated by white spot syndrome virus (WSSV) infection; Mjdap-1 knockdown decreased the virus copy in vivo and the mortality of M. japonicus to WSSV challenge. Conversely, injecting the purified recombinant MjDAP1 protein promoted the amplification of virus in shrimp. Flow cytometric assay showed, the virus infection-induced apoptosis of hemocytes was enhanced by MjDAP1 protein injection and inhibited in MjDAP1 knockdown shrimp. Furthermore, the expression of apoptosisinducing factor (AIF) was regulated by Mjdap-1, but the caspase transcripts were not affected. Our results suggested that MjDAP1 facilitated the amplification of virus in shrimp, which may be attributed to the promotion of hemocyte apoptosis in an AIF-dependent manner. These results provided a new insight into the function of this protein that may be used for virus disease control. (C) 2017 Elsevier Ltd. All rights reserved.