On the nature of dilute aqueous cholesterol suspensions.
On the nature of dilute aqueous cholesterol suspensions.
复制标题
关于稀水性胆固醇悬浮液的性质。
作者：
P. Renshaw;A. Janoff;K. Miller
通常认为胆固醇在水溶液中形成胶束。我们使用各种技术重新评估了这一发现。在10（-8）M以上的所有浓度下，胆固醇均沉淀。更浓溶液的密度梯度分析显示两个条带，其密度对应于结晶无水胆固醇和结晶胆固醇一水合物。通过光学显微镜和相变表征了一水合物。该带也声称含有胶束，但不能检测到二萘嵌苯或二苯基己三烯荧光的增强。一些一水合物晶体可以通过0.7微米过滤器，但不能通过1.5 nm过滤器。当过滤胆固醇浓度低至2.6 X 10（-8）M的溶液时，在后一种过滤器上检测到一水合物晶体。因此，在所有可能出现胶束的条件下，我们都检测到胆固醇一水合物的微晶，我们无法找到支持胆固醇胶束存在的独立证据。
Cholesterol is commonly supposed to form a micelle in aqueous solution. We have reassessed this finding using a variety of techniques. Centrifugation sedimented cholesterol at all concentrations above 10(-8) M. Density gradient analysis of more concentrated solutions revealed two bands whose densities corresponded to crystalline anhydrous cholesterol and crystalline cholesterol monohydrate. The monohydrate was characterized by light microscopy and phase transition. This band is also claimed to contain micelles, but no enhancement of perylene or diphenylhexatriene fluorescence could be detected. Some crystals of monohydrate could pass through 0.7-microns filters but not through 1.5-nm filters. Crystals of monohydrate were detected on the latter filters when solutions with cholesterol concentrations as low as 2.6 X 10(-8) M were filtered. Thus, under all conditions where micelles might be expected, we have detected micro-crystals of cholesterol monohydrate and we can find no independent evidence to support the existence of a cholesterol micelle.
胆汁中胆固醇胆结石的溶解：结晶（无水物和一水物）胆固醇与鹅去氧胆酸盐、熊去氧胆酸盐及其甘氨酸和牛磺酸缀合物的溶解动力学。
影响因子：
6.5
作者：
Igimi,H;Carey,MC
通讯作者：
Carey,MC