Evaluation of the Mean Corpuscular Volume of Peripheral Blood Mononuclear Cells of HIV Patients by a Coulter Counter To Determine Intracellular Drug Concentrations
Evaluation of the Mean Corpuscular Volume of Peripheral Blood Mononuclear Cells of HIV Patients by a Coulter Counter To Determine Intracellular Drug Concentrations
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DOI：
10.1128/aac.01236-10
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发表时间：
2011-06-01
影响因子：
4.9
通讯作者：
Di Perri, Giovanni
中科院分区：
文献类型：
--
作者：
Simiele, Marco;D'Avolio, Antonio;Di Perri, Giovanni
用库尔特计数器测定外周血单个核细胞（PBMCs）的平均红细胞体积（MCV），计算细胞内药物浓度。从190名患者中共收集了574份PBMC样品。MCV为282.9 fl（最小值207.0;最大值354.6），标准差为8.8%。以前的报告经常使用400 fl的MCV固定值，这可能会导致抗病毒药物细胞内浓度的人为低估计值。
The mean corpuscular volume (MCV) of peripheral blood mononuclear cells (PBMCs) was determined by Coulter Counter, and data were used to calculate the intracellular drug concentrations. A total of 574 PBMC samples were collected from 190 patients. The MCV was 282.9 fl (minimum, 207.0; maximum, 354.6), with a standard deviation of 8.8%. Previous reports have often used a fixed value of 400 fl for the MCV, which may result in artificially low estimates of the intracellular concentrations of antivirals.