Effect of dietary choline levels on growth performance, lipid deposition and metabolism in juvenile yellow catfish Pelteobagrus fulvidraco
Effect of dietary choline levels on growth performance, lipid deposition and metabolism in juvenile yellow catfish Pelteobagrus fulvidraco
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DOI：
10.1016/j.cbpb.2016.07.005
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发表时间：
2016-12-01
影响因子：
2.2
通讯作者：
Wu, Kun
中科院分区：
文献类型：
--
作者：
Luo, Zhi;Wei, Chuan-Chuan;Wu, Kun
本试验旨在探讨饲料胆碱水平对黄颡鱼生长性能和脂肪沉积的影响及其机制。饮食胆碱包括三个水平的2392（对照（没有额外的胆碱添加），1156.4和2273.6毫克胆碱每公斤饮食，分别），并喂给黄鲶鱼（平均初始重量：3.45 +/- 0.02克平均值+/-平均值的标准误差（SEM））8周。与对照组和高胆碱组相比，饲料中胆碱含量为1156.4mg·kg ~（-1）时，鱼的增重、特定生长率和摄食量较高，但饲料转化率较低。肝体指数（HSI）和肝脏脂质含量随着饲料胆碱水平的增加而下降。肌肉脂肪含量以胆碱充足组最低，其他两组间差异不显著。肝脏和肌肉中胆碱含量随日粮胆碱水平的增加而增加。饲料胆碱水平显著影响肌肉和肝脏脂质稳态相关基因的mRNA水平，如CTP：磷酸胆碱胞苷酰转移酶a（CCT a）、磷脂酰乙醇胺N-甲基转移酶（PEMT）、微粒体甘油三酯转移蛋白（MTP）、载脂蛋白B（APOB B）、载脂蛋白E（ApoE）和脂蛋白脂肪酶（LPL），且饲料胆碱水平对脂质沉积和代谢的影响具有组织特异性。这些基因在mRNA水平上的不同反应部分解释了饲喂不同胆碱饲料的鱼类肝脏和肌肉中脂质沉积的概况。据我们所知，这是第一次探讨饲料胆碱水平对这些基因的mRNA表达的影响，这为鱼类胆碱营养提供了新的见解。(C)2016 Elsevier Inc. All rights reserved.
The present experiment was conducted to determine the effect and mechanism of dietary choline levels on growth performance and lipid deposition of yellow catfish Pelteobagrus fulvidraco. Dietary choline was included at three levels of 2392 (control (without extra choline addition), 1156.4 and 2273.6 mg choline per kg diet, respectively) and fed to yellow catfish (mean initial weight: 3.45 +/- 0.02 g mean +/- standard errors of mean (SEM)) for 8 weeks. Fish fed the diet containing 1156.4 mg kg(-1) choline showed the higher weight gain (WG), specific growth rate (SGR) and feed intake (FI), but the lower feed conversion rate (FCR), than those in control and highest choline group. Hepatosomatic index (HSI) and hepatic lipid content declined with increasing dietary choline levels. Muscle lipid content was the lowest for fish fed adequate choline diets and showed no significant difference between other two groups. Choline contents in liver and muscle increased with increasing dietary choline levels. Dietary choline levels significantly influenced mRNA levels of genes involved in lipid homeostasis in muscle and liver, such as CTP:phosphocholine cytidylyltransferase a (CCTa), phosphatidylethanolamine N-methyl-transferase (PEMT), microsomal triglyceride transfer protein (MTP), apolipoprotein b (APOBb), apolipoprotein E (ApoE) and lipoprotein lipase (LPL), and effects of dietary choline levels on lipid deposition and metabolism were tissue-specific. Different responses of these genes at the mRNA levels partially explained the profiles of lipid deposition in liver and muscle for fish fed different choline diets. To our knowledge, this is the first to explore the effect of dietary choline level on mRNA expression of these genes, which provides new insights into choline nutrition in fish. (C) 2016 Elsevier Inc. All rights reserved.