LIMITATIONS OF HAIRLESS MOUSE SKIN AS A MODEL FOR INVITRO PERMEATION STUDIES THROUGH HUMAN-SKIN - HYDRATION DAMAGE
LIMITATIONS OF HAIRLESS MOUSE SKIN AS A MODEL FOR INVITRO PERMEATION STUDIES THROUGH HUMAN-SKIN - HYDRATION DAMAGE
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DOI：
10.1111/1523-1747.ep12460958
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发表时间：
1988-04-01
影响因子：
6.5
通讯作者：
BARRY, BW
中科院分区：
文献类型：
--
作者：
BOND, JR;BARRY, BW
无毛小鼠皮肤目前为人体经皮吸收研究提供了一种流行的模型膜。虽然已经证明了两种皮肤类型之间的一些相似之处，但长期水合作用对无毛小鼠皮肤的影响以前没有严格检查过。我们已经在体外测量了在31 ℃下水合的效果。C对无毛小鼠皮肤和人腹部和头皮皮肤对模型极性化合物（水）和脂质材料（己醇）的渗透性的影响。 无毛小鼠皮肤的渗透性急剧上升，特别是对水的渗透性（增加了50倍），而人类皮肤则不稳定。我们还比较了无毛小鼠和人类腹部皮肤的剥离角质层的效果与8-d水合作用的效果。无毛小鼠皮肤的水合作用在消除角质层屏障方面与胶带剥离一样有效，而剥离人类皮肤远比水合作用更具破坏性，这表明长时间的水合作用机械地破坏小鼠皮肤而不是人类皮肤。新鲜和水合组织的组织学检查证实了这一建议。因此，我们建议在长期水合条件下的体外渗透研究期间，不使用无毛小鼠皮肤作为人体组织的模型，即，超过三天。
Hairless mouse skin currently provides a popular model membrane for studies in human percutaneous absorption. Although some similarities between the two skin types have been demonstrated the effects of prolonged hydration on hairless mouse skin have not previously been rigorously examined. We have measured in vitro the effects of hydration at 31.degree. C on the permeabilities of hairless mouse skin and human abdominal and scalp skin to a model polar compound (water) and a lipid material (hexanol). The permeability of hairless mouse skin rose dramatically, especially to water (fiftyfold increase), whereas the human skin was none stable. We also compared the effects of stripping the stratum corneum with the effects of 8-d hydration for hairless mouse and human abdominal skin. Hydration of hairless mouse skin was a effective as tape-stripping in eliminating the stratum corneum barrier, whereas stripping human skin was far more damaging than hydration, suggesting that prolonged hydration mechanically disrupted mouse skin but not human skin. Histological examination of fresh and hydrated tissues confirmed this suggestion. We therefore recommended that hairless mouse skin is not used as a model for human tissue during in vitro permeation studies under conditions of long-term hydration, i.e., greater than three days.