Differential gene expression profiles of the mitochondrial respiratory components in illuminated Arabidopsis leaves.
Differential gene expression profiles of the mitochondrial respiratory components in illuminated Arabidopsis leaves.
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DOI：
10.1093/pcp/pcp090
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发表时间：
2009-08
影响因子：
4.9
通讯作者：
Keisuke Yoshida;K. Noguchi
中科院分区：
文献类型：
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作者：
Keisuke Yoshida;K. Noguchi
植物线粒体在呼吸链中具有多种能量耗散成分。众所周知，这些成分是在几种应力条件下诱发的。在这里，我们检查了拟南芥叶片呼吸成分在高光（HL）条件下的基因表达模式及其调控机制是否不同。暴露于 HL 后，替代氧化酶 (AOX) 基因表达（AOX1a 和 AOX1c）和蛋白质含量升高。除AOX外，HL还诱导其他呼吸基因的表达，包括NDA1、NDB2、NDC1、UCP1、UCP5、COX6b和CI76。 NDB2 与 AOX1a 共表达，但其他 HL 诱导的基因表现出不同的表达模式。使用几种化学物质对光合作用或呼吸的操纵表明，虽然 AOX1a 和 NDB2 的表达主要是通过抑制呼吸链诱导的，但 NDA1 的表达受到光合作用相关活性氧的影响。这些操作并未诱导 AOX1c、NDC1、COX6b 和 CI76 的表达。当植物在高CO(2)环境下暴露于HL时，一些呼吸基因的表达被更强烈地诱导，这表明通过提高光合作用来调节细胞碳状态涉及呼吸基因表达。基于这些结果，我们提出了光照叶子中呼吸基因表达的机制模型。
Plant mitochondria have multiple energy-dissipating components in the respiratory chain. It is known that these components are induced under several stress conditions. Here we examined whether the gene expression pattern and its regulatory mechanism under high light (HL) conditions are different among the respiratory components in Arabidopsis leaves. Alternative oxidase (AOX) gene expression (AOX1a and AOX1c) and amount of protein were elevated after exposure to HL. In addition to AOX, the expression of other respiratory genes, including NDA1, NDB2, NDC1, UCP1, UCP5, COX6b and CI76, was also induced by HL. NDB2 was co-expressed with AOX1a, but other HL-induced genes showed a distinct expression pattern. Manipulation of photosynthesis or respiration using several chemicals revealed that while the expression of AOX1a and NDB2 was mainly induced by inhibition of the respiratory chain, NDA1 expression was affected by photosynthesis-related reactive oxygen species. The expression of AOX1c, NDC1, COX6b and CI76 was not induced by these manipulations. When plants were exposed to HL under a high CO(2) environment, the expression of several respiratory genes was more strongly induced, suggesting that modulations of cellular carbon status by elevated photosynthesis are involved in respiratory gene expression. Based on these results, we propose a mechanistic model of respiratory gene expression in illuminated leaves.