Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques
Comprehensive characterization of MHC class II haplotypes in Mauritian cynomolgus macaques
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DOI：
10.1007/s00251-007-0209-7
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发表时间：
2007-06-01
期刊：
影响因子：
3.2
通讯作者：
O'Connor, David H.
中科院分区：
文献类型：
--
作者：
O'Connor, Shelby L.;Blasky, Alex J.;O'Connor, David H.
目前还没有完全定义的主要组织相容性复合体（MHC）II类遗传学的非人灵长类动物模型。我们最近发现，六个常见的MHC单倍型占基本上所有的MHC多样性食蟹猴（Macaca fascicularis）从毛里求斯岛。在这项研究中，我们采用互补的DNA克隆和测序，以全面表征全长MHC II类等位基因表达的Mafa-DPA，-DPB，-DQA，-DQB，-DRB和-DRB基因座上的六个常见的单倍型。我们描述了34个全长MHC II类等位基因，其中12个是完全新颖的。多态性是明显的，在所有六个位点，包括DPA，一个位点被认为是单态的恒河猴。与其他旧大陆猴相似，马达加斯加食蟹猴（MCM）在DQ和DR位点与人类共享MHC II类等位基因谱系，但不在DP位点。此外，我们确定了广泛的共享MHC II类等位基因之间的MCM和其他非人灵长类动物。这些全长表达的MHC II类等位基因的表征将使研究人员能够产生MHC II类transferent细胞系，四聚体和其他分子试剂，可用于探索MCM中的CD 4 + T淋巴细胞应答。
There are currently no nonhuman primate models with fully defined major histocompatibility complex (MHC) class II genetics. We recently showed that six common MHC haplotypes account for essentially all MHC diversity in cynomolgus macaques (Macaca fascicularis) from the island of Mauritius. In this study, we employ complementary DNA cloning and sequencing to comprehensively characterize full length MHC class II alleles expressed at the Mafa-DPA, -DPB, -DQA, -DQB, -DRA, and -DRB loci on the six common haplotypes. We describe 34 full-length MHC class II alleles, 12 of which are completely novel. Polymorphism was evident at all six loci including DPA, a locus thought to be monomorphic in rhesus macaques. Similar to other Old World monkeys, Mauritian cynomolgus macaques (MCM) share MHC class II allelic lineages with humans at the DQ and DR loci, but not at the DP loci. Additionally, we identified extensive sharing of MHC class II alleles between MCM and other nonhuman primates. The characterization of these full-length-expressed MHC class II alleles will enable researchers to generate MHC class II transferent cell lines, tetramers, and other molecular reagents that can be used to explore CD4+ T lymphocyte responses in MCM.