Chitooligosaccharides induce apoptosis in human myeloid leukemia HL-60 cells
Chitooligosaccharides induce apoptosis in human myeloid leukemia HL-60 cells
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DOI：
10.1016/j.bmcl.2012.08.030
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发表时间：
2012-10-01
影响因子：
2.7
通讯作者：
Park, Pyo-Jam
中科院分区：
文献类型：
--
作者：
Kim, Eun-Kyung;Je, Jae-Young;Park, Pyo-Jam
在这项研究中，我们提出了一种新型的使用杂壳寡糖的抗癌药物。为了探讨杂多酸作为抗癌药物的可能性，我们制备了9种相对分子质量较高(90，75和50-COS I，5个接近10 kDa)，中等相对分子质量(90，75和50-COS II，1个接近5 kDa)和低分子(90，75和50-III，小于1 kDa)的杂多酚，并用流式细胞仪和形态分析研究了它们对HL-60细胞的抗癌作用。结果表明，脱乙酰度相对较高、相对分子质量较低的90-COS III具有最高的抗癌活性，杂化COS的抗癌活性与其脱乙酰度和相对分子质量有关。(C)2012爱思唯尔有限公司。保留所有权利。
In this study we propose a novel anticancer agent using hetero-chitooligosaccharide (hetero-COS). To examine the possibility of the hetero-COS as a anticancer agent, we prepared nine kinds of hetero-COS with relatively higher molecular weights (90, 75 and 50-COS I, 5 similar to 10 kDa), medium molecular weights (90, 75 and 50-COS II, 1 similar to 5 kDa), and lower molecular weights (90, 75 and 50-III, below 1 kDa), and their anticancer properties were investigated on HL-60 cells using flow cytometry and morphological analysis. The results obtained indicate that 90-COS III, which is relatively higher degree of deacetylation and lower molecular weights, showed the highest anticancer activity, and the data showed the anticancer property of the hetero-COSs depended on their degree of deacetylation values and molecular weight. (C) 2012 Elsevier Ltd. All rights reserved.