DNA-TEMPLATE EFFECT ON RNA SPLICING - 2 COPIES OF THE SAME GENE IN THE SAME NUCLEUS ARE PROCESSED DIFFERENTLY
DNA-TEMPLATE EFFECT ON RNA SPLICING - 2 COPIES OF THE SAME GENE IN THE SAME NUCLEUS ARE PROCESSED DIFFERENTLY
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DOI：
10.1002/j.1460-2075.1991.tb04910.x
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发表时间：
1991-11-01
期刊：
影响因子：
11.4
通讯作者：
BABISS, LE
中科院分区：
文献类型：
--
作者：
ADAMI, G;BABISS, LE
许多细胞和病毒基因是含有多种剪接选择的复杂转录单位的一部分。在腺病毒复制周期的过程中，取决于感染的阶段，单个基因的不同剪接版本占主导地位。这对于几种腺病毒基因是正确的。在本文中，我们显示的病毒E1B转录单位，剪接位点的使用调节这一过程。该系统中可变剪接的变化不依赖于转录基因的序列。非腺病毒基因，如SV40早期区和多瘤早期区，通常很少或不显示剪接RNA产物形成的调节，在插入腺病毒基因组后，mRNA的产生受到调节。其他研究表明，腺病毒中的E1 B剪接调节是顺式效应。使用两个可辨别的病毒基因组的交错感染导致早期和晚期基因组存在于同一细胞核中的情况。两个基因组都不能将其受调控的剪接模式强加于另一个基因组，这表明病毒基因剪接中的转换线索并不直接依赖于反式作用剪接因子的全局变化。这表明E1B基因RNA加工变化的信号与DNA模板的状态或其在核亚区室中的定位有关。
Many cellular and viral genes are parts of complex transcription units containing multiple splicing choices. During the course of an adenoviral replicative cycle, different spliced versions of a single gene predominate, depending on the stage of infection. This is true for several adenoviral genes. In this paper we show for the viral E1B transcription unit that splice site usage regulates this process. The change in alternative splicing in this system does not depend on the sequence of the transcribed genes. Non-adenoviral genes, such as the SV40 early region and the polyoma early region, which normally show little or no regulation of spliced RNA product formation, become regulated for mRNA production after insertion into the adenoviral genome. Additional studies show that E1B splicing regulation in adenovirus is a cis effect. Staggered infections using two discernable viral genomes resulted in a situation where both early and late genomes exist in the same nucleus. Neither genome was able to impose its regulated splicing pattern on the other, indicating that the cue for the switch in viral gene splicing is not directly dependent on global changes in trans-acting splicing factors. This suggests a model where the signal for changes in RNA processing for the E1B gene is linked to the state of the DNA template or its localization within nuclear subcompartments.