Seasonal DNA Methylation Variation in the Flat Tree Oyster Isognomon Alatus from a Mangrove Ecosystem in North Biscayne Bay, Florida
Seasonal DNA Methylation Variation in the Flat Tree Oyster Isognomon Alatus from a Mangrove Ecosystem in North Biscayne Bay, Florida
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DOI：
10.2983/035.038.0108
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发表时间：
2019-04
影响因子：
1.3
通讯作者：
V. Suárez-Ulloa;C. Rivera-Casas;M. Michel;J. Eirín-López
中科院分区：
文献类型：
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作者：
V. Suárez-Ulloa;C. Rivera-Casas;M. Michel;J. Eirín-López
表观遗传学分析为更好地理解环境响应的机制及其在不同生态系统的驯化和适应过程中的作用提供了一种新兴的方法。将环境表观遗传学研究扩展到更广泛的生态和环境相关生物，将提高预测生态和进化过程的能力，并通过“表观遗传足迹”分析促进对生物监测生物的应激暴露进行回顾性评估。为此，本研究监测了北比斯坎湾（佛罗里达州北迈阿密）红树林生态系统2年时间内非生物参数（温度、盐度、pH值和水平能见度）的时空变化。随后，将获得的数据与扁平树牡蛎Isognomon alatus的表观遗传修饰（全球全基因组DNA甲基化水平）进行比较，后者被用作实验地点的哨兵模式生物。获得的结果显示，在时间DNA甲基化模式中存在一定程度的季节性，这似乎主要与温度和水平能见度的变化有关。这些结果构成了首次在自然环境中对海洋生物进行时空表观遗传分析的长期研究，为评估环境表观遗传分析的生物监测潜力奠定了初步基础。
ABSTRACT Epigenetic analyses constitute an emerging approach for better understanding of the mechanisms underlying environmental responses and their role during acclimatization and adaptation across diverse ecosystems. The expansion of environmental epigenetic studies to a broader range of ecologically and environmentally relevant organisms will enhance the capability to forecast ecological and evolutionary processes, as well as to facilitate a retrospective assessment of stress exposures in biomonitor organisms through “epigenetic footprinting” analyses. With such purpose, the present study monitored spatial and temporal variation in abiotic parameters (temperature, salinity, pH, and horizontal visibility) over a 2-y period in a mangrove ecosystem located in North Biscayne Bay (North Miami, FL). The obtained data were subsequently compared with epigenetic modifications (global genome-wide DNA methylation levels) in the flat tree oyster Isognomon alatus, used as a sentinel model organism across experimental sites. The obtained results revealed a certain level of seasonality in temporal DNA methylation patterns, which seem to be primarily associated with changes in temperature and horizontal visibility. These results constitute the first long-term study combining spatial and temporal epigenetic analyses in a marine organism in its natural environment, laying the initial groundwork to assess the biomonitoring potential of environmental epigenetic analyses.