Thermosensitization by sulfhydryl compounds of exponentially growing Chinese hamster cells.
Thermosensitization by sulfhydryl compounds of exponentially growing Chinese hamster cells.
复制标题
指数生长的中国仓鼠细胞的巯基化合物的热敏化。
作者：
D. Kapp;G. Hahn
研究了各种巯基化合物对加热至37-43 ℃的中国仓鼠细胞（HA 1）的指数生长单层培养物存活的影响。在室温或37度下无毒或毒性最小的半胱胺浓度在升高的温度下变得毒性越来越大，大大增强了在不存在半胱胺的情况下单独加热产生的杀伤。这种高血压诱导的细胞杀伤的增强随着半胱胺浓度的增加、半胱胺暴露的持续时间的增加和温度的增加而增加。在16 mM半胱胺存在下，在43 ℃下加热1小时的同步化中国仓鼠细胞的研究表明，热杀伤的增强发生在细胞周期的所有阶段。同样，高血压诱导的细胞杀伤的增强被视为异步细胞暴露于2-氨基乙基异硫脲溴化物和半胱氨酸，但不同的巯基化合物的效果的大小不同。
The effect of various sulfhydryl compounds on the survival of exponentially growing monolayer cultures of Chinese hamster cells (HA1) heated to temperatures of 37-43 degrees was examined. Concentrations of cysteamine which were nontoxic or minimally toxic at room temperature or 37 degrees became increasingly toxic at elevated temperatures, greatly potentiating the killing produced by heat alone in the absence of cysteamine. This enhancement of hyperthermia-induced cell killing increased with increasing cysteamine concentration, increasing duration of cysteamine exposure, and increasing temperature. Studies with synchronized Chinese hamster cells heated at 43 degrees for 1 hr in the presence of 16 mM cysteamine demonstrated that the potentiation of heat killing occurred in all phases of the cell cycle. Similarly, enhancement of hyperthermia-induced cell killing was seen for asynchronous cells exposed to 2-amino-ethylisothiourium bromides and cysteine, but the magnitude of the effect differed for the various sulfhydryl compounds.