Non-invasive quantification of cerebral blood flow for rats by microPET imaging of 15O labelled water: the application of a cardiac time-activity curve for the tracer arterial input function.
Non-invasive quantification of cerebral blood flow for rats by microPET imaging of 15O labelled water: the application of a cardiac time-activity curve for the tracer arterial input function.
复制标题
通过 15O 标记水的 microPET 成像对大鼠脑血流量进行无创定量：心脏时间-活动曲线在示踪动脉输入函数中的应用。
DOI：
10.1097/00006231-200510000-00009
复制
发表时间：
2005
影响因子：
1.5
通讯作者：
Fox,PeterT
中科院分区：
文献类型：
--
作者：
Yee,Seong-Hwan;Jerabek,PaulA;Fox,PeterT
目的利用正电子发射断层扫描(PET)进行活体脑血流量(CBF)定量测量，通常采用有创动脉采血方法来确定动脉输入功能(AIF)。方法10只雄性大鼠(Fischer 344,247~290g)，在α-氯醛糖复合麻醉(30 mg·kg−1·h−1，静脉滴注)下，采用动态正电子发射计算机断层扫描技术，采用静脉推注H2 15 O的方法，测量大鼠脑血流量。取而代之的是，使用从动态PET成像获得的心脏时间-活动曲线(TAC)来确定AIF。为了验证这项技术，还通过计算颈动脉注射后示踪剂(H215O)的洗脱率来测量CBF。用两种独立的方法测量CBF，同时将体温调节和维持在两个不同的水平(直肠温度分别为32±1和37±1℃)。结果低温(32℃)条件下脑血流量(m l·100g−-1·m in-−-1)分别为45.2±6.0 5(静脉)和4 7.4±8.6 4(颈动脉内)，常温(37℃)分别为5 5.1±4.88和5 4.4±4.6 0。两种方法具有较好的一致性(r=0.70)。结论我们建立的小动物心脏TAC分析技术可用于基于PET的活体成像技术的无创性脑血流定量。
ObjectiveIn-vivo quantitative cerebral blood flow (CBF) measurement using positron emission tomography (PET) has typically employed invasive arterial blood sampling procedure to determine the arterial input function (AIF). The present study was performed to provide a non-invasive quantitative CBF measurement technique for rats using a dedicated animal PET.MethodsCBF was measured in 10 male rats (Fischer 344, 247–290 g) under α-chloralose anesthesia (30 mg· kg− 1· h− 1, intravenous infusion) by dynamic PET imaging employing the intravenous bolus injection of H 2 15 O. Unlike other conventional PET methods, no arterial blood sampling was employed. Instead, a cardiac time–activity curve (TAC) obtained from the dynamic PET imaging was used to determine the AIF. For the validation of this technique, CBF was also measured by calculating the washout rate of the tracer (H 2 15 O) following an intracarotid bolus injection. CBF measurements by two independent methods were done while modulating and maintaining the body temperature at two different levels (32±1 and 37±1 C by the rectal temperature). Two methods were compared by the linear regression analysis.ResultsCBF (ml· 100 g− 1· min− 1) values (mean±SD) were 45.2±6.05 (intravenous) and 47.4±8.64 (intracarotid) at the hypothermic condition (32 C), and 55.1±4.88 (intravenous) and 54.4±4.60 (intracarotid) at the normothermic condition (37 C). There was a good agreement between the two methods (r= 0.70).ConclusionsOur cardiac TAC analysis technique for small animals can be used for the non-invasive quantification of CBF using the PET-based in-vivo imaging technique.