Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast
Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast
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DOI：
10.1073/pnas.93.9.3870
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发表时间：
1996-04-30
影响因子：
11.1
通讯作者：
Maresca, B
中科院分区：
文献类型：
--
作者：
Carratu, L;Franceschelli, S;Maresca, B
当细胞在 37°C 下热激时，添加饱和脂肪酸 (SEA) 会诱导热激 (HS) mRNA 转录的强烈增加，而用不饱和脂肪酸 (UFA) 处理会降低或消除 37°C 下的 HS 基因转录水平。荚膜组织胞浆菌 (Histoplasma capsulatum) 的 Delta(9)-去饱和酶基因 (Ole1) 的转录在细胞中上调，其基因产物负责 UFA 的合成。温度敏感应变。我们发现，当酿酒酵母的 L8-14C 突变体（其 Ole1 基因被破坏）与其自身的 Ole1 编码区（在其自身启动子或 H. capsulatum 的 Ole1 启动子控制下）互补时，EIS 基因转录水平取决于启动子的活性。互补菌株线粒体膜的荧光各向异性对应于所使用的 Ole1 启动子的不同活性。我们认为，SFA/UFA 比率和膜脂蛋白复合物的扰动与快速温度变化的感知有关，并且在 HS 条件下，对预先存在的膜物理状态的干扰会导致诱导 HS 基因转录的信号转导。
Addition of a saturated fatty acid (SEA) induced a strong increase in heat shock (HS) mRNA transcription when cells were heat-shocked at 37 degrees C, whereas treatment with an unsaturated fatty acid (UFA) reduced or eliminated the level of HS gene transcription at 37 degrees C. Transcription of the Delta(9)-desaturase gene (Ole1) of Histoplasma capsulatum, whose gene product is responsible for the synthesis of UFA, is up-regulated in a temperature-sensitive strain. We show that when the L8-14C mutant of Saccharomyces cerevisiae, which has a disrupted Ole1 gene, is complemented with its own Ole1 coding region under control of its own promoter or Ole1 promoters of H. capsulatum, the level of EIS gene transcription depends on the activity of the promoters. Fluorescence anisotropy, of mitochondrial membranes of complemented strains corresponded to the different activity of the Ole1 promoter used. We propose that the SFA/UFA ratio and perturbation of membrane lipoprotein complexes are involved in the perception of rapid temperature changes and under HS conditions disturbance of the preexisting membrane physical state causes transduction of a signal that induces transcription of HS genes.