Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells.
Nanoparticle-antagomiR based targeting of miR-31 to induce osterix and osteocalcin expression in mesenchymal stem cells.
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DOI：
10.1371/journal.pone.0192562
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Berry CC
中科院分区：
文献类型：
--
作者：
McCully M;Conde J;V Baptista P;Mullin M;Dalby MJ;Berry CC
间充质干细胞是一种多能的成体干细胞，能够生成骨、软骨和脂肪，因此目前正被用于再生医学。在考虑成骨时，在化学诱导(如分化介质)和物理诱导(如材料硬度、纳米拓扑学)方面取得了进展，靶向已建立的早期转录因子或调控因子，如Runx2或骨形态发生蛋白，并促进更多的细胞致力于骨特异性分化。最近的研究强调了microRNAs在谱系承诺和末端分化中的作用。在本研究中，具有稳定短单链RNA的金纳米颗粒被用来在体外将MIR-31反义体输送到成骨前细胞和原代人MSCs。结果显示，阻断miR-31在第7天导致两种细胞中Osterix蛋白的增加，在第21天骨钙素增加，提示MSC成骨。此外，还注意到antagomiR序列方向很重要，事实证明，5个碱基的阅读方向比3个碱基更有效。这项研究强调了miRNA antagomiR标记的纳米颗粒在再生医学中作为新疗法提供的潜力。
Mesenchymal stem cells are multipotent adult stem cells capable of generating bone, cartilage and fat, and are thus currently being exploited for regenerative medicine. When considering osteogenesis, developments have been made with regards to chemical induction (e.g. differentiation media) and physical induction (e.g. material stiffness, nanotopography), targeting established early transcription factors or regulators such as runx2 or bone morphogenic proteins and promoting increased numbers of cells committing to osteo-specific differentiation. Recent research highlighted the involvement of microRNAs in lineage commitment and terminal differentiation. Herein, gold nanoparticles that confer stability to short single stranded RNAs were used to deliver MiR-31 antagomiRs to both pre-osteoblastic cells and primary human MSCs in vitro. Results showed that blocking miR-31 led to an increase in osterix protein in both cell types at day 7, with an increase in osteocalcin at day 21, suggesting MSC osteogenesis. In addition, it was noted that antagomiR sequence direction was important, with the 5 prime reading direction proving more effective than the 3 prime. This study highlights the potential that miRNA antagomiR-tagged nanoparticles offer as novel therapeutics in regenerative medicine.
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miR-31、Runx2 和 Satb2 调节环对骨间充质干细胞成骨分化的影响
影响因子：
4
作者：
Deng, Yuan;Wu, Si;Fan, Xianqun
通讯作者：
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影响因子：
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作者：
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KAZHDAN, I
DOI：
10.1016/j.bbrc.2011.10.117
发表时间：
2011-12-09
影响因子：
3.1
作者：
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通讯作者：
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影响因子：
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作者：
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