Archiving and absolute quantitation of solutes separated by single charged droplet coulomb explosion
Archiving and absolute quantitation of solutes separated by single charged droplet coulomb explosion
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DOI：
10.1021/ac048113e
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发表时间：
2005-06-01
影响因子：
7.4
通讯作者：
Agnes, GR
中科院分区：
文献类型：
--
作者：
Bakhoum, SFW;Bogan, MJ;Agnes, GR
电喷雾（ES）离子源依赖于低挥发性溶质向气相的转移，这是电雾化产生的带电液滴库仑爆炸事件的结果。本文介绍了两种通过在电平衡中悬浮的带有净电荷的单液滴的库仑爆炸分离化合物的存档方法。我们将爆炸分离的化合物分为喷出的物质（包括子代液滴）和残留在主要残留物中的物质。这种方法的潜力体现在(i)溶质状态的定性表征，分离物质中的水相和沉淀态，以及（ii）通过这种事件分离的溶质的绝对定量。对于含有5 × 10(7)个直径为20纳米的荧光球的液滴，其第一次遇到库仑爆炸事件导致1.70%的荧光球喷射出来。在ES离子源中将低挥发性溶质转移到气相的过程中，获取有关各个步骤的详细信息的能力对于开发ES质谱应用中的绝对定量策略至关重要。
The electrospray (ES) ion source relies on the transfer of low-volatility solutes to the gas phase as an outcome of coulomb explosion events of charged droplets generated by electrical atomization. Introduced here are two methods for archiving compounds separated by coulomb explosion of single droplets having net charge that had been levitated in an electrodynamic balance. We categorized compounds separated by the explosion as either material ejected, including progeny droplets, or the material retained in the main residue. The potential for this methodology is illustrated by (i) qualitative characterization of solute states, aqueous versus precipitated in the separated material, and (ii) absolute quantitation of solutes separated by such an event. For a droplet containing 5 x 10(7) 20-nm-diameter fluospheres, its first encountered coulomb explosion event resulted in the ejection of 1.70% of them. The capability to acquire such detailed information regarding the individual steps in the process of transferring low-volatility solutes to the gas phase in an ES ion source is essential to develop strategies for absolute quantitation in applications of ES mass spectrometry.