Cell type specific expression of Follistatin-like 1 (Fstl1) in mouse embryonic lung development
Cell type specific expression of Follistatin-like 1 (Fstl1) in mouse embryonic lung development
复制标题
卵泡抑素样 1 (Fstl1) 在小鼠胚胎肺发育中的细胞类型特异性表达
DOI：
10.1007/s10735-018-9780-5
复制
发表时间：
2018-08-01
影响因子：
3.2
通讯作者：
Ning, Wen
中科院分区：
文献类型：
--
作者：
Liu, Xue;Liu, Yingying;Ning, Wen
卵泡抑素样-1（Fstl1）是一种分泌型糖蛋白，可被TGF-β 1上调。为了更好地研究Fstl1在肺发育中的功能，我们使用他莫昔芬诱导的Fstl1报告小鼠品系，以细胞类型特异性的方式检测了Fstl1在发育中的肺中的表达。我们的研究结果表明，Fstl1在发育中的肺中的囊状期普遍表达。在E18.5，Fstl1表达在大多数类型的间充质细胞中是稳健的，包括气道周围的气道平滑肌细胞、血管平滑肌细胞、内皮细胞和来自血管的血管周细胞，但在远端肺泡囊中不是PDGFR α（+）成纤维细胞。同时，在上皮细胞中观察到相对弱和散发的Fstl1表达信号，包括近端气道中的俱乐部细胞亚群和远端气道中的少数II型肺泡上皮细胞。我们的数据有助于理解Fstl1在肺发育和肺部疾病发病机制中的关键作用。
Follistatin like-1 (Fstl1) is a secreted glycoprotein and can be up-regulated by TGF-beta 1. To better study the function of Fstl1 in lung development, we examined Fstl1 expression in the developing lung, in a cell type specific manner, using a tamoxifen inducible Fstl1-reporter mouse strain. Our results show that Fstl1 is ubiquitously expressed at saccular stage in the developing lung. At E18.5, Fstl1 expression is robust in most type of mesenchymal cells, including airway smooth muscle cells surrounding airways, vascular smooth muscle cells, endothelial cells, and vascular pericytes from blood vessel, but not PDGFR alpha(+) fibroblasts in the distal alveolar sacs. Meanwhile, relative weak and sporadic signals of Fstl1 expression are observed in epithelium, including a subgroup of club cells in proximal airways and a few type II alveolar epithelial cells in distal airways. Our data help to understand the critical role of Fstl1 in lung development and lung disease pathogenesis.