Screening the cultivar and processing factors based on the flavonoid profiles of dry teas using principal component analysis
Screening the cultivar and processing factors based on the flavonoid profiles of dry teas using principal component analysis
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基于干茶黄酮谱的主成分分析筛选品种和加工因子
DOI：
10.1016/j.jfca.2017.12.016
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发表时间：
2018-04-01
影响因子：
4.3
通讯作者：
Liang, Yue-Rong
中科院分区：
文献类型：
--
作者：
Zheng, Xin-Qiang;Nie, Ying;Liang, Yue-Rong
本研究筛选了品种和加工方法对不同茶类黄酮含量的影响。以“九龙泡”、“浙农902”、“金选”和“黄金桂”4个茶树品种为试材，分别加工成绿色茶、乌龙茶和红茶。采用超高效液相色谱-二极管阵列检测器-串联质谱（UHPLC-DAD-MS/MS）结合固体火箭发动机（SRM）模式同时测定茶叶中13种黄酮醇苷和8种儿茶素类化合物的含量。同一类型茶叶中黄酮醇苷类的品种间差异大于儿茶素类。红茶中总黄酮醇苷和总儿茶素含量分别比同品种绿色茶低14.2%和28.8%，低82.9%和84.6%。杨梅苷、单苷、三苷和儿茶素易受发酵影响。主成分分析结果表明，干茶的黄酮醇苷类化合物很好地区分了茶树品种，而不是制造过程，而红茶的儿茶素类化合物的发酵过程中区分。因此，茶树品种是一个重要的因素，以考虑消费决策的干茶，特别是非发酵和半发酵茶，由于显着品种间差异的黄酮醇苷。
This study screened the effects of cultivar and processing method on the flavonoid profiles of different teas. Four tea cultivars 'Jiulongpao', 'Zhenong 902', 'Jinxuan' and 'Huangjingui' were respectively processed into green tea, oolong tea and black tea. The concentrations of 13 flavonol glycosides and 8 catechins in teas were simultaneously determined by UHPLC-DAD-MS/MS in combination with SRM mode. The intercultivar difference of flavonol glycosides in the same type of teas was greater than that of catechins. The contents of total flavonol glycosides and total catechins in black teas were respectively 14.2% similar to 28.8% and 82.9% similar to 84.6% lower than those of green teas made from the same cultivar. Myricetin glycosides, mono-glycosides, tri-glycosides and catechins are vulnerable to fermentation. Principal component analysis results indicated that the flavonol glycoside profiles of dry teas were well discriminated by tea plant cultivar rather than manufacturing procedure, whereas the catechin profiles of black teas were distinguished by fermentation process. Thus, tea plant cultivar is an important factor to be considered for consumption decision on dry teas, especially non-fermented and semi-fermented teas, due to remarkable intercultivar difference in flavonol glycosides.