Trace Quantitative Analysis by Mass Spectrometry
Trace Quantitative Analysis by Mass Spectrometry
复制标题
质谱痕量定量分析
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
R. Bethem
中科院分区：
文献类型：
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作者：
R. Boyd;C. Basic;R. Bethem
前言。致谢。1测量、尺寸和单位。1.1介绍。1.2国际单位制（SI）。1.3质谱法中的“质荷比”，1.4 SI基本量测量的可实现精度，1.5质谱法痕量定量的分子质量极限，1.6关键概念总结，2行业工具，1.经典工具，2.1简介，2.2分析和内部标准：参考材料。2.3分析天平。2.4体积的测量和配药。2.5校准溶液的制备。2.6定量分析校准方法介绍。2.7关键概念总结。3行业工具色谱理论。3.1绪论。3.2化学分离的一般原理。3.3重要概念总结。3.4色谱板理论。3.5色谱中的非平衡效应：van Deemter方程。3.6梯度洗脱。3.7毛细管电泳和毛细管电色谱。附录3.1色谱洗脱平板理论方程的推导。附录3.2平板理论洗脱方程由泊松形式转化为高斯形式。附录3.3 Snyder的梯度洗脱理论简介。第三章中使用的符号列表。4贸易工具分离实用性。4.1简介。4.2分析物与基质。4.3萃取与清理：样品制备方法。4.4色谱实用性。4.5关键概念总结。附录4.1色谱检测器的响应：浓度与质量通量依赖。5行业工具。色谱-质谱的界面和离子源。5.1介绍。5.2在色谱仪和源之间可能需要离散接口的离子源。5.3不需要离散接口的离子源。5.4基于离子迁移率的源-分析仪接口。5.5关键概念总结。5.1附录5.1:MALDI分析的样品制备方法。定量用质谱分析仪：通过m / z值分离离子。6.1介绍。6.2质谱分析仪操作模式和串联质谱法。6.3离子在电场和磁场中的运动。6.4质谱分析仪。6.5离子的活化和解离。6.6真空系统。6.7关键概念总结。附录6.1电场和磁场与带电粒子的相互作用。附录6.2泄漏检测。附录6.3第六章中使用的符号列表。7行业工具VI.离子检测和数据处理。7.1介绍。7.2法拉第杯探测器。7.3电子倍增器。7.4后探测器电子学。7.5关键概念总结。8行业工具VII：校准、测量和抽样统计。8.1介绍。8.2单变量数据：确定准确性和精度的工具和测试。8.3双变量数据：回归和相关的工具和检验。8.4检测和定量限。8.5校准和测量：系统和随机误差。8.6异质矩阵抽样统计。8.7关键概念总结。附录8.1简要统计术语表。附录8.2校准方法讨论中使用的符号。9方法开发和目的适应度。9.1介绍。9.2目的适应度和管理不确定性。9.3分析人员和客户之间的问题：检查风险。9.4制定策略。9.5方法开发。9.6矩阵效应。9.7污染和遗留。9.8建立最终方法。10受控实验室环境中的方法验证和样品分析。10.1介绍。10.2方法验证。10.3验证的实施。10.4适合目的的方法和验证示例。10.5验证的样品分析。10.6文件编制。10.7可追溯性。11来自文献的示例。11.1导论。11.2食品污染物。11.3水中人为污染物。11.4环境持久性污染物的GC-MS分析。11.5生物分析应用。11.6定量蛋白质组学。11.7内源性分析物分析。跋。参考文献。索引。
Preface. Acknowledgements. 1 Measurement, Dimensions and Units. 1.1 Introduction. 1.2 The International System of Units (SI). 1.3 'Mass-to-Charge Ratio' in Mass Spectrometry. 1.4 Achievable Precision in Measurement of SI Base Quantities. 1.5 Molecular Mass Limit for Trace Quantitation by Mass Spectrometry. 1.6 Summary of Key Concepts. 2 Tools of the Trade I. The Classical Tools. 2.1 Introduction. 2.2 Analytical and Internal Standards: Reference Materials. 2.3 The Analytical Balance. 2.4 Measurement and Dispensing of Volume. 2.5 Preparation of Solutions for Calibration. 2.6 Introduction to Calibration Methods for Quantitative Analysis. 2.7 Summary of Key Concepts. 3 Tools of the Trade II. Theory of Chromatography. 3.1 Introduction. 3.2 General Principles of Chemical Separations. 3.3 Summary of Important Concepts. 3.4 Plate Theory of Chromatography. 3.5 Nonequilibrium Effects in Chromatography: the van Deemter Equation. 3.6 Gradient Elution. 3.7 Capillary Electrophoresis and Capillary Electrochromatography. Appendix 3.1 Derivation of the Plate Theory Equation for Chromatographic Elution. Appendix 3.2 Transformation of the Plate Theory Elution Equation from Poisson to Gaussian Form. Appendix 3.3 A Brief Introduction to Snyder's Theory of Gradient Elution. List of Symbols Used in Chapter 3. 4 Tools of the Trade III. Separation Practicalities. 4.1 Introduction. 4.2 The Analyte and the Matrix. 4.3 Extraction and Clean-Up: Sample Preparation Methods. 4.4 Chromatographic Practicalities. 4.5 Summary of Key Concepts. Appendix 4.1 Responses of Chromatographic Detectors: Concentration vs Mass-Flux Dependence. 5 Tools of the Trade IV. Interfaces and Ion Sources for Chromatography-Mass Spectrometry. 5.1 Introduction. 5.2 Ion Sources that can Require a Discrete Interface Between Chromatograph and Source. 5.3 Ion Sources not Requiring a Discrete Interface. 5.4 Source-Analyzer Interfaces Based on Ion Mobility. 5.5 Summary of Key Concepts. 5.1 Appendix 5.1: Methods of Sample Preparation for Analysis by MALDI. 6 Tools of the Trade V. Mass Analyzers for Quantitation: Separation of Ions by m / z Values. 6.1 Introduction. 6.2 Mass Analyzer Operation Modes and Tandem Mass Spectrometry. 6.3 Motion of Ions in Electric and Magnetic Fields. 6.4 Mass Analyzers. 6.5 Activation and Dissociation of Ions. 6.6 Vacuum Systems. 6.7 Summary of Key Concepts. Appendix 6.1 Interaction of Electric and Magnetic Fields with Charged Particles. Appendix 6.2 Leak Detection. Appendix 6.3 List of Symbols Used in Chapter 6. 7 Tools of the Trade VI. Ion Detection and Data Processing. 7.1 Introduction. 7.2 Faraday Cup Detectors. 7.3 Electron Multipliers. 7.4 Post-Detector Electronics. 7.5 Summary of Key Concepts. 8 Tools of the Trade VII: Statistics of Calibration, Measurement and Sampling. 8.1 Introduction. 8.2 Univariate Data: Tools and Tests for Determining Accuracy and Precision. 8.3 Bivariate Data: Tools and Tests for Regression and Correlation. 8.4 Limits of Detection and Quantitation. 8.5 Calibration and Measurement: Systematic and Random Errors. 8.6 Statistics of Sampling of Heterogeneous Matrices. 8.7 Summary of Key Concepts. Appendix 8.1 A Brief Statistics Glossary. Appendix 8.2 Symbols Used in Discussion of Calibration Methods. 9 Method Development and Fitness for Purpose. 9.1 Introduction. 9.2 Fitness for Purpose and Managing Uncertainty. 9.3 Issues Between Analyst and Client: Examining What's at Stake. 9.4 Formulating a Strategy. 9.5 Method Development. 9.6 Matrix Effects. 9.7 Contamination and Carryover. 9.8 Establishing the Final Method. 10 Method Validation and Sample Analysis in a Controlled Laboratory Environment. 10.1 Introduction. 10.2 Method Validation. 10.3 Conduct of the Validaton. 10.4 Examples of Methods and Validations Fit for Purpose. 10.5 Validated Sample Analysis. 10.6 Documentation. 10.7 Traceability. 11 Examples from the Literature. 11.1 Introduction. 11.2 Food Contaminants. 11.3 Anthropogenic Pollutants in Water. 11.4 GC-MS Analyses of Persistent Environmental Pollutants. 11.5 Bioanalytical Applications. 11.6 Quantitative Proteomics. 11.7 Analysis of Endogenous Analytes. Epilog. References. Index.