Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae
Comparison of tests for the detection of circulating filarial antigen (Og4C3-ELISA and AD12-ICT) and ultrasound in diagnosis of lymphatic filariasis in individuals with microfilariae
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DOI：
10.1590/s0074-02762009000400015
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发表时间：
2009-07-01
期刊：
影响因子：
--
通讯作者：
Furtado, André
中科院分区：
文献类型：
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作者：
Rocha, Abraham;Braga, Cynthia;Furtado, André
20世纪90年代，随着三种新的替代工具的出现，丝虫病的诊断取得了重大进展：超声和使用两种单克隆抗体Og 4C 3和AD 12-ICT卡检测循环抗原的试验。本研究旨在确定这些方法中哪一种对感染诊断最敏感。通过所有三种技术检测了通过夜间静脉血样诊断的256名携带微丝蚴（1- 15，679 MF/mL）的男性。循环丝虫抗原检测符合率为100%，正确检出阳性者246/256（96.69%），而超声仅检出186/256（73.44%）。在循环抗原检测中，ICT卡是最简便的班氏丝虫带菌者鉴定方法。它很容易执行，实用和快速。
Significant advances were made in the diagnosis of filariasis in the 1990s with the emergence of three new alternative tools: ultrasound and tests to detect circulating antigen using two monoclonal antibodies, Og4C3 and AD12-ICT-card. This study aimed to identify which of these methods is the most sensitive for diagnosis of infection. A total of 256 individuals, all male and carrying microfilariae (1-15,679 MF/mL), diagnosed by nocturnal venous blood samples, were tested by all three techniques. The tests for circulating filarial antigen concurred 100% and correctly identified 246/256 (96.69%) of the positive individuals, while ultrasound detected only 186/256 (73.44%). Of the circulating antigen tests, ICT-card was the most convenient method for identification of Wuchereria bancrofti carriers. It was easy to perform, practical and quick.