Amacrine Cell Subtypes Differ in Their Intrinsic Neurite Growth Capacity
Amacrine Cell Subtypes Differ in Their Intrinsic Neurite Growth Capacity
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DOI：
10.1167/iovs.13-12691
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发表时间：
2013-11-01
影响因子：
4.4
通讯作者：
Goldberg, Jeffrey L.
中科院分区：
文献类型：
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作者：
Kunzevitzky, Noelia J.;Willeford, Kevin T.;Goldberg, Jeffrey L.
目的。无长突细胞神经突模式已在体内进行了广泛研究，并且已在哺乳动物视网膜中鉴定出 30 多个具有不同形态的亚群。然而，尚不清楚复杂的无长突细胞形态是由内在决定的，还是由外在信号发出信号，或两者兼而有之。在这里，我们将大鼠无长突细胞亚群从其视网膜邻居和神经胶质衍生因子中纯化出来，以询问其内在神经突生长能力的问题。在体外对无长突细胞具有强营养性的确定培养基中，我们通过特定标记物的表达来表征无长突细胞亚群的存活和神经突生长。结果。我们发现一系列无长突细胞亚型标记物受到发育调节，并在出生后早期发育过程中开启。在培养的无长突细胞的相似部分中观察到亚型标志物的表达与体内观察到的一样，并且随着培养时间的推移而维持。总体而言，无长突细胞神经突的生长遵循与出生后视网膜神经节细胞非常相似的原理，但胚胎视网膜神经节细胞表现出不同的特征，与其快速轴突生长有关。令人惊讶的是，体外研究的三个无长突细胞亚群定量和定性地概括了它们在体内具有的不同形态。结论。我们的数据表明，培养的无长突细胞对其识别的体内亚型保持内在保真度，此外，细胞自主的内在因素有助于神经突模式的调节。
PURPOSE. Amacrine cell neurite patterning has been extensively studied in vivo, and more than 30 subpopulations with varied morphologies have been identified in the mammalian retina. It is not known, however, whether the complex amacrine cell morphology is determined intrinsically, is signaled by extrinsic cues, or both.METHODS. Here we purified rat amacrine cell subpopulations away from their retinal neighbors and glial-derived factors to ask questions about their intrinsic neurite growth ability. In defined medium strongly trophic for amacrine cells in vitro, we characterized survival and neurite growth of amacrine cell subpopulations defined by expression of specific markers.RESULTS. We found that a series of amacrine cell subtype markers are developmentally regulated, turning on through early postnatal development. Subtype marker expression was observed in similar fractions of cultured amacrine cells as was observed in vivo, and was maintained with time in culture. Overall, amacrine cell neurite growth followed principles very similar to those in postnatal retinal ganglion cells, but embryonic retinal ganglion cells demonstrated different features, relating to their rapid axon growth. Surprisingly, the three subpopulations of amacrine cells studied in vitro recapitulated quantitatively and qualitatively the varied morphologies they have in vivo.CONCLUSIONS. Our data suggest that cultured amacrine cells maintain intrinsic fidelity to their identified in vivo subtypes, and furthermore, that cell-autonomous, intrinsic factors contribute to the regulation of neurite patterning.