OsSNDP1, a Sec14-nodulin domain-containing protein, plays a critical role in root hair elongation in rice
OsSNDP1, a Sec14-nodulin domain-containing protein, plays a critical role in root hair elongation in rice
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DOI：
10.1007/s11103-013-0033-4
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发表时间：
2013-05-01
影响因子：
5.1
通讯作者：
Han, Chang-Deok
中科院分区：
文献类型：
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作者：
Huang, Jin;Kim, Chul Min;Han, Chang-Deok
水稻是在水田上种植的。因此，与生长在排水土壤中的根毛相比，表皮层的水稻根毛暴露于不同的氮种、有机酸和金属离子的氧化还原状态。为了鉴定在根毛生长中起重要作用的基因，采用正向遗传学方法筛选短根毛突变体。利用图谱克隆和测序技术，鉴定并分离了一株短根毛突变体。该突变源于OsSNDP1 （Oryza sativa sec14 - nodin domain protein）的单氨基酸替换，该突变与拟南芥COW1/AtSFH1序列高度同源，并编码一个磷脂酰肌醇转移蛋白（PITP）。通过与Atsfh1突变体的互补实验，我们证实OsSNDP1参与了根毛的生长。利用冷冻扫描电镜进一步表征Ossndp1突变对根毛形态的影响。根毛伸长区和成熟区形态发生异常。根毛多分枝，因节凸起而形状不规则。许多表皮细胞还产生圆顶状的根毛，这表明根毛的伸长在早期就停止了。这些研究表明，pitp介导的磷脂信号和代谢对水稻根毛伸长至关重要。
Rice is cultivated in water-logged paddy lands. Thus, rice root hairs on the epidermal layers are exposed to a different redox status of nitrogen species, organic acids, and metal ions than root hairs growing in drained soil. To identify genes that play an important role in root hair growth, a forward genetics approach was used to screen for short-root-hair mutants. A short-root-hair mutant was identified and isolated by using map-based cloning and sequencing. The mutation arose from a single amino acid substitution of OsSNDP1 (Oryza sativa Sec14-nodulin domain protein), which shows high sequence homology with Arabidopsis COW1/AtSFH1 and encodes a phosphatidylinositol transfer protein (PITP). By performing complementation assays with Atsfh1 mutants, we demonstrated that OsSNDP1 is involved in growth of root hairs. Cryo-scanning electron microscopy was utilized to further characterize the effect of the Ossndp1 mutation on root hair morphology. Aberrant morphogenesis was detected in root hair elongation and maturation zones. Many root hairs were branched and showed irregular shapes due to bulged nodes. Many epidermal cells also produced dome-shaped root hairs, which indicated that root hair elongation ceased at an early stage. These studies showed that PITP-mediated phospholipid signaling and metabolism is critical for root hair elongation in rice.