HAIRY ROOT NODULATION OF CASUARINA-GLAUCA - A SYSTEM FOR THE STUDY OF SYMBIOTIC GENE-EXPRESSION IN AN ACTINORHIZAL TREE
HAIRY ROOT NODULATION OF CASUARINA-GLAUCA - A SYSTEM FOR THE STUDY OF SYMBIOTIC GENE-EXPRESSION IN AN ACTINORHIZAL TREE
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DOI：
10.1094/mpmi-8-0532
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发表时间：
1995-07-01
影响因子：
3.5
通讯作者：
BOGUSZ, D
中科院分区：
文献类型：
--
作者：
DIOUF, D;GHERBI, H;BOGUSZ, D
本研究的目的是建立一种快速生产木麻黄转基因放线根根瘤的系统。使用携带 p35S-gusA-int 基因构建体的发根农杆菌菌株 A4RS 在 3 周龄青冈菌幼苗的下胚轴上诱导毛状根，伤后 3 周，切除原始根系，将未转化芽上由转基因根组成的复合植株转移至试管中，接种 Frankia，放线菌 转化根上形成的根瘤具有未转化根瘤的固氮酶活性和形态。通过荧光和组织化学分析检测转基因根和根瘤中的β-葡萄糖醛酸酶（GUS）活性。结果表明，用该根转化系统产生的转基因根瘤可以促进放线根树共生固氮的分子研究。
The purpose of this study was to establish a fast system for producing transgenic actinorhizal root nodules of Casuarina glauca. Agrobacterium rhizogenes strain A4RS carrying the p35S-gusA-int gene construct was used to induce hairy roots on hypocotyls of 3-week-old C. glauca seedlings, Three weeks after wounding, the original root system was excised, and composite plants consisting of transgenic roots on untransformed shoots were transferred to test tubes to be inoculated with Frankia, The actinorhizal nodules formed on transformed roots had the nitrogenase activity and morphology of untransformed nodules. beta-Glucuronidase (GUS) activity was examined in transgenic roots and nodules by fluorometric and histochemical assays. The results indicate that transgenic nodules generated with this root transformation system could facilitate the molecular study of symbiotic nitrogen fixation in actinorhizal trees.