Atypical Hemolytic Listeria innocua Isolates Are Virulent, albeit Less than Listeria monocytogenes
Atypical Hemolytic Listeria innocua Isolates Are Virulent, albeit Less than Listeria monocytogenes
复制标题
DOI：
10.1128/iai.00758-18
复制
发表时间：
2019-04-01
影响因子：
3.1
通讯作者：
Lecuit, Marc
中科院分区：
文献类型：
--
作者：
Moura, Alexandra;Disson, Olivier;Lecuit, Marc
无害李斯特菌被认为是一种非致病性李斯特菌。天然非典型溶血L.已经报道了无害的分离物，但是还没有详细地表征。在这里，我们报告了两个已知的天然溶血性L。无毒枝全基因组测序证实了李斯特菌致病岛（LIPI）的单核细胞增多性李斯特菌物种的特征的存在。功能分析表明，LIPI-1和inlA基因转录，相应的基因产物表达和功能。使用体外和体内试验，我们表明，非典型溶血L。无害的是有毒的，可以主动穿过肠上皮，并全身扩散到肝脏和脾脏，尽管程度低于参考L。单核细胞增生EGDe菌株。虽然人类接触溶血性L。无害的可能是罕见的，这些发现是重要的食品安全和公共卫生。一些L. innocua进化枝支持L.单核细胞增多症和L.无害。
Listeria innocua is considered a nonpathogenic Listeria species. Natural atypical hemolytic L. innocua isolates have been reported but have not been characterized in detail. Here, we report the genomic and functional characterization of representative isolates from the two known natural hemolytic L. innocua clades. Wholegenome sequencing confirmed the presence of Listeria pathogenicity islands (LIPI) characteristic of Listeria monocytogenes species. Functional assays showed that LIPI-1 and inlA genes are transcribed, and the corresponding gene products are expressed and functional. Using in vitro and in vivo assays, we show that atypical hemolytic L. innocua is virulent, can actively cross the intestinal epithelium, and spreads systemically to the liver and spleen, albeit to a lesser degree than the reference L. monocytogenes EGDe strain. Although human exposure to hemolytic L. innocua is likely rare, these findings are important for food safety and public health. The presence of virulence traits in some L. innocua clades supports the existence of a common virulent ancestor of L. monocytogenes and L. innocua.