HIGH-LEVELS OF CORTICOTROPIN-RELEASING HORMONE IMMUNOACTIVITY IN MATERNAL AND FETAL PLASMA DURING PREGNANCY
HIGH-LEVELS OF CORTICOTROPIN-RELEASING HORMONE IMMUNOACTIVITY IN MATERNAL AND FETAL PLASMA DURING PREGNANCY
复制标题
DOI：
10.1210/jcem-63-5-1199
复制
发表时间：
1986-11-01
影响因子：
5.8
通讯作者：
FRANTZ, AG
中科院分区：
文献类型：
--
作者：
GOLAND, RS;WARDLAW, SL;FRANTZ, AG
本文测定了31例妊娠妇女和6例非妊娠妇女的血浆促肾上腺皮质激素释放激素免疫活性(CRHI)，以及40例足月胎儿脐带血浆的CRHI。6名非妊娠妇女或6名妊娠早期妇女的血浆中未检出CRHI(<；44pg/ml)。平均血浆CRHI逐渐升高至58。+-。18及270+-。孕中期和孕晚期分别为68pg/ml，产后24小时内再次检测不到。40例脐带血浆CRHI平均值为136.+-。16pg/ml，胎儿和母体血浆的凝胶过滤显示，CRHI的大部分洗脱位置与人工合成的人CRH相同。CRHI与脐带血浆β-内啡肽和ACTH均无显著相关性，提示CRHI可能不是胎儿血浆中β-内啡肽和ACTH释放的主要原因。10对母婴同时测得的母血和脐血CRHI之间存在密切的相关性(r=0.82)，支持母胎循环中CRHI的共同来源。胎儿和母体CRHI的胎盘来源之一是脐静脉CRHI浓度高于脐动脉CRHI浓度，以及分娩后母体血浆中CRHI的消失。我们的结论是，在妊娠期间，胎盘将大量CRHI分泌到母体和胎儿的循环中，这可能是妊娠期母体和胎儿下丘脑-垂体-肾上腺轴的重要调节因子。
Corticotropin-releasing hormone immunoactivity (CRHi) was measured in the plasma of 31 pregnant women and 6 nonpregnant women as well as in the umbilical cord plasma of 40 term fetuses. CRHi was not detectable (< 44 pg/ml) in the plasma of 6 nonpregnant women or in 6 women in the first trimester of pregnancy. Mean plasma CRHi rose progressively to 58 .+-. 18 and 270 .+-. 68 pg/ml during the second and third trimesters, respectively, and again became undetectable within 24 h after delivery. Mean CRHi in 40 umbilical cord plasma samples was 136 .+-. 16 pg/ml. Gel filtration of both fetal and maternal plasma showed that the majority of the CRHi eluted in the same position as synthetic human CRH. There was no significant correlation between CRHi and either .beta.-endorphin or ACTH in umbilical cord plasma, suggesting that this CRHi may not be primarily responsible for the release of .beta.-endorphin and ACTH into fetal plasma at delivery. A close correlation (r = 0.82) was found between simultaneously obtained maternal and umbilical cord plasma CRHi in 10 maternal-fetal pairs, supporting a common source for this peptide in maternal and fetal circulation. A placental source for fetal and maternal CRHi was suggested by the finding of a higher CRHi concentration in the umbilical vein than in the umbilical artery and by the disappearance of this peptide from maternal plasma after delivery. We conclude that a large amount of CRHi is secreted by the placenta into both the maternal and fetal circulation during pregnancy and suggest that this may be an important modulator of the matenral and fetal hypothalamic-pituitary-adrenal axis during gestation.