Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions
Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions
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DOI：
10.1016/j.bios.2007.04.009
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发表时间：
2007-09-30
影响因子：
12.6
通讯作者：
Tengvall, Pentti
中科院分区：
文献类型：
--
作者：
Hansson, Kenny M.;Johansen, Knut;Tengvall, Pentti
设计了一种基于表面等离子体共振(SPR)的流室装置，用于实时检测富血小板血浆(PRP)和全血中的凝血和血小板黏附。该系统允许在流室的6圈长度内检测表面相互作用。在凝血酶沉积到流室入口附近的传感器表面的一段后，在该位置下游检测到凝血，对应于7~8mRIU(7~8 ng/mm(2))的SPR信号。未经修饰的对照表面诱导凝血的速度慢3.5倍。缓冲液中的血小板与金和纤维蛋白原涂层表面的粘附量分别为1.25mRIU和1.66mRIU。在平行实验中，用PRP和全血在甲基化或涂有von Willebrand因子(VWF)、纤维蛋白原或胶原的表面上获得的SPR反应与荧光显微镜观察到的血小板粘附性很好地吻合。目前配备SPR检测的流室系统是一种很有前途的研究生理流动条件下凝血事件和血细胞黏附的工具，并允许监测全血的短程表面过程。(C)2007 Elsevier B.V.保留所有权利。
A surface plasmon resonance (SPR) based flow chamber device was designed for real time detection of blood coagulation and platelet adhesion in platelet rich plasma (PRP) and whole blood. The system allowed the detection of surface interactions throughout the 6 turn length of the flow chamber. After deposition of thromboplastin onto a section of the sensor surface near the inlet of the flow chamber, coagulation was detected downstream of this position corresponding to a SPR signal of 7 to 8 mRIU (7 to 8 ng/mm(2)). A nonmodified control surface induced coagulation 3.5 times slower. Platelet adhesion to gold and fibrinogen coated surfaces in the magnitude of 1.25 and 1.66 mRIU was also shown with platelets in buffer, respectively. SPR responses obtained with PRP and whole blood on surfaces that were methylated or coated with von Willebrand factor (vWF), fibrinogen, or collagen, coincided well with platelet adhesion as observed with fluorescence microscopy in parallel experiments. The present SPR detection equipped flow chamber system is a promising tool for studies on coagulation events and blood cell adhesion under physiological flow conditions, and allows monitoring of short-range surface processes in whole blood. (C) 2007 Elsevier B.V. All rights reserved.