Exercise training prevents the perivascular adipose tissue-induced aortic dysfunction with metabolic syndrome
Exercise training prevents the perivascular adipose tissue-induced aortic dysfunction with metabolic syndrome
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DOI：
10.1016/j.redox.2019.101285
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发表时间：
2019-09-01
期刊：
影响因子：
11.4
通讯作者：
Chantler, Paul D.
中科院分区：
文献类型：
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作者：
DeVallance, Evan;Branyan, Kayla W.;Chantler, Paul D.
本研究的目的是确定运动训练对改善代谢综合征中胸血管周围脂肪组织 (tPVAT) 表型（炎症、氧化应激和蛋白酶体功能）的效果及其对主动脉功能的后续作用。方法：瘦和肥胖（代谢综合征模型）Zucker 大鼠（n=8/组）接受 8 周的对照条件或 跑步机锻炼（最大速度的 70%，每天 1 小时，每周 5 天）。干预结束时，除去 tPVAT 并制备条件培养基。将清洁后的主动脉连接到力传感器上，以评估在存在或不存在 tPVAT 条件介质的情况下内皮依赖性和独立性扩张。评估了tPVAT基因表达、炎症/氧化表型和蛋白酶体功能。结果：主要发现Ex诱导：（1）米色样、抗炎tPVAT表型； (2) tPVAT 中 (NO)-N-中心点的丰度更高； (3) tPVAT氧化剂产生减少； (4) 改善的 tPVAT 蛋白酶体功能。关于主动脉功能，与对照组相比，运动的瘦人和肥胖组的内皮依赖性扩张更大（p < 0.05）。瘦控制 tPVAT 改善主动脉舒张，而肥胖控制 tPVAT 减少主动脉舒张。相比之下，肥胖的 Ex-tPVAT 会增加主动脉扩张，而瘦的 Ex-tPVAT 不会影响主动脉扩张。 结论：总体而言，运动对肥胖 tPVAT 的影响最为显着，反映出氧化负荷减少、炎症减少和蛋白酶体功能改善的环境发生变化。运动对 tPVAT 微环境的这种有益变化可能在调节 8 周运动后代谢综合征主动脉功能的改善中发挥了重要作用。
The aim of the study was to determine the effects of exercise training on improving the thoracic perivascular adipose tissue (tPVAT) phenotype (inflammation, oxidative stress, and proteasome function) in metabolic syndrome and its subsequent actions on aortic function.Methods: Lean and obese (model of metabolic syndrome) Zucker rats (n=8/group) underwent 8-weeks of control conditions or treadmill exercise (70% of max speed, 1 h/day, 5 days/week). At the end of the intervention, the tPVAT was removed and conditioned media was made. The cleaned aorta was attached to a force transducer to assess endothelium-dependent and independent dilation in the presence or absence of tPVATconditioned media. tPVAT gene expression, inflammatory /oxidative phenotype, and proteasome function were assessed.Results: The main findings were that Ex induced: (1) a beige-like, anti-inflammatory tPVAT phenotype; (2) a greater abundance of (NO)-N-center dot in tPVAT; (3) a reduction in tPVAT oxidant production; and (4) an improved tPVAT proteasome function. Regarding aortic function, endothelium-dependent dilation was greater in exercised lean and obese groups vs. controls (p < 0.05). Lean control tPVAT improved aortic relaxation, whereas obese control tPVAT decreased aortic relaxation. In contrast, the obese Ex-tPVAT increased aortic dilation, whereas the lean Ex-tPVAT did not affect aortic dilation.Conclusion: Overall, exercise had the most dramatic impact on the obese tPVAT reflecting a change towards an environment with less oxidant load, less inflammation and improved proteasome function. Such beneficial changes to the tPVAT micro-environment with exercise likely played a significant role in mediating the improvement in aortic function in metabolic syndrome following 8 weeks of exercise.