Chemical cleavage of proteins in solution.
Chemical cleavage of proteins in solution.
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DOI：
10.1002/0471140864.ps1104s40
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发表时间：
2005-06-01
影响因子：
--
通讯作者：
Denslow, Nancy D
中科院分区：
文献类型：
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作者：
Crimmins, Dan L;Mische, Sheenah M;Denslow, Nancy D
Described in this unit are five basic protocols that are widely used for specific and efficient chemical cleavage of proteins in solution. Cyanogen bromide (CNBr) cleaves at methionine (Met) residues; BNPS-skatole cleaves at tryptophan (Trp) residues; formic acid cleaves at aspartic acid-proline (Asp-Pro) peptide bonds; hydroxylamine cleaves at asparagine-glycine (Asn-Gly) peptide bonds, and 2-nitro-5-thiocyanobenzoic acid (NTCB) cleaves at cysteine (Cys) residues. Because the above loci are at relatively low abundance in most proteins, digestion with these agents will yield relatively long peptides.
