A microarray study of chronic unpredictable mild stress rat blood serum with electro-acupuncture intervention
A microarray study of chronic unpredictable mild stress rat blood serum with electro-acupuncture intervention
复制标题
电针干预慢性不可预知轻度应激大鼠血清微阵列研究
DOI：
10.1016/j.neulet.2016.05.054
复制
发表时间：
2016-08-03
影响因子：
2.5
通讯作者：
Liu, Ping
中科院分区：
文献类型：
--
作者：
Duan, Dong Mei;Dong, Xianzhe;Liu, Ping
在本研究中，我们利用微阵列分析研究了慢性不可预测轻度应激（CUMS）大鼠抑郁和电针（EA）干预后microRNA （miRNA）表达的变化。结果表明，EA干预显著改善了CUMS大鼠的杂交次数、饲养次数、蔗糖偏好和体重等行为指标。微阵列分析显示，共有153个差异表达mirna受CUMS调控，EA干预后180个差异表达mirna的表达发生改变。在这些miRNAs中，2个miRNAs被CUMS显著上调，4个miRNAs被CUMS显著下调。EA干预后，4个mirna显著上调，12个mirna显著下调。CUMS后miR-383-5p和miR-764-5p表达上调，EA干预后miR-383-5p和miR-764-5p表达下调。进一步分析表明，miR-383-5p和miR-764-5p预测了1260个可能的靶基因，涉及97条通路和137条基因本体（GO）。在这些通路和GO中，约有20条通路和21条GO与抑郁有关。miR-383-5p和miR-764-5p的变化提示EA可能通过促进神经萎缩、抑制神经元异常凋亡及其他相关信号通路发挥其治疗抑郁症的作用。总之，本研究丰富了对抑郁症病理过程的认识，揭示了EA治疗抑郁症的可能机制。2016爱思唯尔爱尔兰有限公司版权所有。
In the present study, we investigated the changes of microRNA (miRNA) expression upon depression and electro-acupuncture (EA) intervention in chronic unpredictable mild stress (CUMS) rats using microarray analysis. Results showed that EA intervention remarkably improved behavioral indexes in terms of crossing number, rearing number, sucrose preference and body weight of CUMS rats. Microarray analysis revealed that a total of 153 differentially expressed miRNAs were regulated by CUMS, and the expression of 180 differentially expressed miRNAs was changed after EA intervention. Among these miRNAs, two miRNAs were significantly up-regulated and four miRNAs were significantly down-regulated by CUMS. Moreover, four miRNAs were significantly up-regulated and 12 miRNAs were significantly down regulated after EA intervention. The expressions of miR-383-5p and miR-764-5p were up-regulated after CUMS, while their expressions were down-regulated by EA intervention. Further analysis showed that 1260 possible target genes were predicted for miR-383-5p and miR-764-5p, and 97 pathways and 137 gene ontology (GO) were involved. Among these pathways and GO, about 20 pathways and 21 GO were related to depression. Changes of miR-383-5p and miR-764-5p indicated that EA might exert its therapeutic effect on depression through promoting the neurotrophy and inhibiting the abnormal apoptosis of neurons as well as other correlative signal pathways. In conclusion, our present study enriched the understanding of pathological process of depression and revealed possible mechanisms of EA on depression. (C) 2016 Elsevier Ireland Ltd. All rights reserved.