Extended Spectrum β-Lactamases among Gram-Negative Bacterial Isolates from Clinical Specimens in Three Major Hospitals in Northern Jordan
Extended Spectrum β-Lactamases among Gram-Negative Bacterial Isolates from Clinical Specimens in Three Major Hospitals in Northern Jordan
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约旦北部三家主要医院临床标本中革兰氏阴性细菌分离株中的广谱 β-内酰胺酶
DOI：
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发表时间：
2009
影响因子：
3.4
通讯作者：
A. Shurman
中科院分区：
文献类型：
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作者：
R. Batchoun;S. Swedan;A. Shurman
背景和目标。超广谱β-内酰胺酶（ESBL）的产生在世界范围内呈上升趋势，除E.埃希菌和pneumoniae肺炎are acquiring获取this character字符. ESBL的产生可通过自动化、E-test、双纸片扩散（DDD）和PCR检测。本研究旨在确定革兰阴性杆菌临床分离株产ESBL的流行率，并评价头孢噻肟、头孢唑肟、氨曲南、头孢曲松和头孢泊肟对克拉维酸盐检测产ESBL的有效性。方法.采用DDD法对472株经标准方法鉴定的革兰阴性菌进行产ESBL试验。结果108/472株（22.9%）为产ESBL菌株，在三级医院中流行。88.2%的E. clopidium，K.肺炎克雷伯氏菌占28.6%;催产素C. freundii，占A. acceticus和10.8%的E.大肠杆菌产ESBL。DDD试验表明，不同头孢菌素在检测所有产ESBL菌时的效力存在一定差异。加入头孢唑肟片可提高试验的有效性。结论：产ESBL菌在我院住院患者中普遍存在，涉及克雷伯菌属和埃希菌属以外的属，头孢唑肟的加入提高了DDD试验检测ESBL的有效性。
Background and Objectives. Extended spectrum β-lactamase (ESBL) production is increasing all over the world, and organisms other than E. coli and K. pneumoniae are acquiring this character. ESBL production is detectable by automation, E-test, double disk diffusion (DDD), and PCR. This study aimed to determine the prevalence of ESBL production among clinical isolates of gram-negative rods, and to evaluate the effectiveness of augmentation of clavunate with Cefotaxime, Ceftazoxime, Aztreonam, Ceftriaxone, and Cefpodoxime in detecting ESBL production. Methods. 472 clinical gram-negative isolates identified by standard methods were tested for ESBL-production by (DDD) method using six cephalosporins and amoxicillin-clavulinate discs. Results. 108/472 (22.9%) of the isolates were ESBL producers, and were prevalent in tertiary care hospitals. 88.2% of E. cloacae, 71.4% of K. pneumoniae, 28.6% of K. oxytoca, 12.5% of C. freundii, 11.1% of A. calcoacceticus, and 10.8% of E. coli were ESBL producers. The DDD test demonstrated some variations in the efficacy of the different cephalosporins in detecting all the ESBL producers. The inclusion of ceftizoxime discs increased the efficacy of the test. It is concluded that ESBL-producing bacteria were prevalent among our hospitalized patients, and involved genera other than Klebsiella and Escherichia, and the inclusion of ceftizoxime increased the efficacy of ESBL detection by the DDD test.
他唑巴坦是一种有效的灭活剂，可抑制某些抑制剂耐药的 A 类 β-内酰胺酶。
影响因子：
2.1
作者：
Bonomo,RA;Rudin,SA;Shlaes,DM
通讯作者：
Shlaes,DM