Leydig cells of the human testis possess astrocyte and oligodendrocyte marker molecules
Leydig cells of the human testis possess astrocyte and oligodendrocyte marker molecules
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DOI：
10.1078/0065-1281-00630
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发表时间：
2002-01-01
影响因子：
2.5
通讯作者：
Holstein, AF
中科院分区：
文献类型：
--
作者：
Davidoff, MS;Middendorff, R;Holstein, AF
已经确定，人睾丸的间质细胞具有神经内分泌特性，因此是弥漫性神经内分泌（paradion）系统的成员。在本研究中，我们研究了成年大鼠睾丸间质细胞是否（51-86岁）和发展中国家（在妊娠的第15周和第36周之间）人睾丸对神经胶质细胞特异性抗原是免疫阳性的，所述神经胶质细胞特异性抗原例如神经胶质纤维酸性蛋白（GFAP）、半乳糖苷（GalC）、环2 ′，3 ′-核苷酸-3 ′-磷酸二酯酶（CNE），A2 B5-抗原（A2 B5）和O-4-抗原（O-4）。用蛋白质印迹和斑点印迹分析，分别发现GFAP，CN-β，GalC，A_2B_5和O_4在整个睾丸和Leydig细胞蛋白提取物的成年男子。相应的免疫组化研究表明，这些抗原存在于睾丸间质细胞的细胞质中的成年睾丸和睾丸在产前发育。观察到单个抗原的染色强度的一些差异可能取决于单个细胞的功能和/或发育阶段。此外，GFAP-，GalC-和CNPase-immunohistochemistry被发现在许多支持细胞的曲细精管。此外，一些结缔组织细胞（区室化细胞或Co细胞）的管间隙显示免疫阳性的CNE 4，A2 B5和GaIC。结果表明，人睾丸间质细胞，除了它们的内分泌，神经元和神经内分泌功能，具有星形胶质细胞和少突胶质细胞的质量，从而显示多能细胞的质量。间质细胞可能分化为神经系统发育中细胞的特征性表型。此外，建立免疫组化的相似性是一致的假设，胎儿和出生后的Leydig细胞是共同的起源。
It has been established, that Leydig cells of the human testis possess neuroendocrine properties and are therefore a member of the diffuse neuroendocrine (paraneuron) system. In the present study, we examined whether Leydig cells of adult (51-86 year of age) and developing (between the 15(th) and 36(th) week of gestation) human testes are immunopositive for glial cell-specific antigens such as glial fibfillary acidic protein (GFAP), galactocerebroside (GalC), cyclic 2',3'-nucleotide-3'-phosphodiesterase (CNPase), A2B5-antigen (A2B5) and O-4-antigen (O-4). With the use of Western blots and dot blot analyses, respectively, GFAP, CNPase, GalC, A2B5 and O4 were found in whole testes and Leydig cell protein extracts of adult men. Corresponding immunohistochemical studies revealed presence of these antigens in the cytoplasm of Leydig cells both of adult testes and testes during prenatal development. Some differences in staining intensity of single antigens were observed probably depending on the functional and/or developmental stage of the single cells. In addition, GFAP-, GalC- and CNPase-immunopositivity was found in numerous Sertoli cells of the seminiferous tubules. Moreover, some connective tissue cells (compartmentalizing cells or Co-cells) of the intertubular space showed immunopositivity for CNPase, A2B5 and GaIC. The results obtained show that Leydig cells of the human testis, in addition to their endocrine, neuronal and neuroendocrine features, possess qualities of both astrocytes and oligodendrocytes and thus show qualities of multipotential cells. Leydig cells probably differentiate to a phenotype that is characteristic for cells in the developing nervous system. Furthermore, the established immunohistochemical similarities are consistent with the assumption that foetal and postnatal Leydig cells are of common origin.