Identification of UV-B radiation responsive microRNAs and their target genes in chrysanthemum (Chrysanthemum morifolium Ramat) using high-throughput sequencing
Identification of UV-B radiation responsive microRNAs and their target genes in chrysanthemum (Chrysanthemum morifolium Ramat) using high-throughput sequencing
复制标题
利用高通量测序鉴定菊花 (Chrysanthemum morifolium Ramat) 中 UV-B 辐射响应性 microRNA 及其靶基因
DOI：
10.1016/j.indcrop.2020.112484
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发表时间：
2020-09-01
影响因子：
5.9
通讯作者：
Xu，Maojun
中科院分区：
文献类型：
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作者：
Yang，Yanjun;Guo，Jiena;Xu，Maojun
MicroRNAs(MiRNA)在紫外线-B(UV-B)诱导植物体内生物活性成分积累过程中起着关键作用。菊花是一种广受欢迎的观赏草本植物，具有多种生物活性，但其miRNAs及其在UV-B诱导活性成分积累中的作用尚不清楚。高通量miRNA测序技术用于鉴定对UV-B辐射响应的miRNAs。以UV-B辐射下不同时间采收的菊花‘小杨菊’叶片为材料，构建了4个文库。共鉴定出42个经典家族的245个miRNAs。在UV-B辐射下，137个miRNAs表现出差异表达，其中包括71个已知miRNAs和67个新miRNAs。此外，对于80个已知的和85个新的miRNAs，预测了涉及不同生物学过程的1779个独特的靶基因。3-磷酸甘油醛脱氢酶、丙酮酸激酶和葡聚糖内切-1，3-β-D-葡萄糖苷酶分别被预测为miR396f-5p、PC-5p-294053_21和miR397a的潜在靶基因。在已鉴定的靶基因中，5个参与绿原酸和类黄酮生物合成的关键基因被预测为MIR4367-P5、PC-3P-40855_284、MIR393b-P5和PC-3P-114893_93的潜在靶基因。与活性成分生物合成相关的miRNAs的表达受到UV-B辐射的显著影响，表明UV-B辐射对菊花活性成分的积累有很强的影响。有趣的是，一些与类黄酮生物合成相关的miRNA，如PC-3P-114893_93和MIR4367-P5，都受到短期UV-B辐射的抑制，这表明UV-B对类黄酮积累的贡献依赖于低剂量的UV-B辐射。
MicroRNAs (miRNA) play a key roles in ultraviolet-B (UV-B) induced accumulation of bioactive ingredients in plants. Chrysanthemum (Chrysanthemum morifoliumRamat) is a popular ornamental herb with numerous biological activities, but its miRNAs and their roles in UV-B-induced active ingredient accumulation remain unexplored. High-throughput miRNA sequencing technology was used to identify miRNAs responsive to UV-B radiation. Four libraries were constructed fromChrysanthemum‘Xiaoyangju’ leaves harvested at different time points under UV-B radiation. In total, 245 miRNAs belonging to 42 classical families were identified. Under UV-B treatment, 137 miRNAs showed differential expression, including 71 known and 67 novel miRNAs,. In addition, 1,779 unique target genes involving diverse biological processes were predicted for 80 known and 85 novel miRNAs. Three key glycolysis genes,Glyceraldehyde-3-Phosphate Dehydrogenase,Pyruvate KinaseandGlucan Endo-1,3-beta-D-Glucosidase, were predicated as potential target genes of miR396f-5p, PC-5p-294053_21 and miR397a, respectively. Among identified target genes, five key genes involved in the biosynthesis of chlorogenic acids and flavonoids were predicated as potential targets genes of MIR4367-p5, PC-3p-40855_284, MIR393b-p5, and PC-3p-114893_93. The expression of identified miRNAs relating to bioactive ingredient biosynthesis was significantly affected by UV-B radiation, suggesting a strong effect of UV-B radiation on bioactive ingredient accumulation inChrysanthemum. Interestingly, several miRNAs related to flavonoids biosynthesis such as PC-3p-114893_93 and MIR4367-p5, were inhibited by short-term UV-B radiation, suggesting that the contribution of UV-B to flavonoids accumulation depends on a low dose of UV-B radiation.