Open source software for quantification of cell migration, protrusions, and fluorescence intensities.
Open source software for quantification of cell migration, protrusions, and fluorescence intensities.
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用于定量细胞迁移，突起和荧光强度的开源软件。
DOI：
10.1083/jcb.201501081
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发表时间：
2015-04-13
期刊：
影响因子：
--
通讯作者：
Way M
中科院分区：
文献类型：
--
作者：
Barry DJ;Durkin CH;Abella JV;Way M
ADAPT是ImageJ插件，可用于延时视频的快速全细胞分析，从而提供有关细胞形态、膜速度和细胞周边任何感兴趣的荧光蛋白的时间变化的数据，如细胞泡的形态学表征所示。细胞迁移经常伴随着细胞形态学（形态动力学）在一系列空间和时间尺度上的变化。尽管最近在成像技术的进步，应用无偏的计算图像分析方法的形态动力学定量是罕见的。例如，使用记波图的手动分析仍然很常见，这通常是由于缺乏用户友好的自动化工具。我们现在描述设计用于自动量化细胞迁移和形态动力学的软件。ADAPT作为开源平台ImageJ的插件实现，能够快速自动分析跨多个细胞的迁移和膜突起，以及相关的荧光标记蛋白质。我们通过量化不同细胞外基质上细胞群体迁移率的变化来证明该软件的能力。我们还表明，ADAPT可以检测和形态轮廓丝状伪足。最后，我们已经使用ADAPT编译了一个公正的描述，一个“典型的”水泡形成的质膜和量化的影响Arp 2/3复合物抑制水泡退缩。
ADAPT is an ImageJ plug-in that can be used for rapid whole-cell analysis of time-lapse videos, thereby providing data on cell morphology, membrane velocity, and temporal changes in any fluorescent protein of interest at the cell periphery, as exemplified by the morphological characterization of cellular blebs. Cell migration is frequently accompanied by changes in cell morphology (morphodynamics) on a range of spatial and temporal scales. Despite recent advances in imaging techniques, the application of unbiased computational image analysis methods for morphodynamic quantification is rare. For example, manual analysis using kymographs is still commonplace, often caused by lack of access to user-friendly, automated tools. We now describe software designed for the automated quantification of cell migration and morphodynamics. Implemented as a plug-in for the open-source platform, ImageJ, ADAPT is capable of rapid, automated analysis of migration and membrane protrusions, together with associated fluorescently labeled proteins, across multiple cells. We demonstrate the ability of the software by quantifying variations in cell population migration rates on different extracellular matrices. We also show that ADAPT can detect and morphologically profile filopodia. Finally, we have used ADAPT to compile an unbiased description of a “typical” bleb formed at the plasma membrane and quantify the effect of Arp2/3 complex inhibition on bleb retraction.
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DOI：
10.1083/jcb.200802081
发表时间：
2008-06-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fackler OT;Grosse R
通讯作者：
Grosse R
影响因子：
30.3
作者：
Humphries, Ashley C.;Dodding, Mark P.;Way, Michael
通讯作者：
Way, Michael
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
3.4
作者：
Charras, Guillaume T.;Coughlin, Margaret;Mahadevan, L.
通讯作者：
Mahadevan, L.
DOI：
10.1073/pnas.1207968109
发表时间：
2012-09-04
影响因子：
11.1
作者：
Bergert, Martin;Chandradoss, Stanley D.;Paluch, Ewa
通讯作者：
Paluch, Ewa