Restriction of gene expression in B lymphocytes and their progeny. III. Endogenous IgA and IgM on the membranes of different plasma cell precursors.
Restriction of gene expression in B lymphocytes and their progeny. III. Endogenous IgA and IgM on the membranes of different plasma cell precursors.
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DOI：
10.1084/jem.140.4.966
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发表时间：
1974-10-01
期刊：
影响因子：
--
通讯作者：
Cebra JJ
中科院分区：
文献类型：
--
作者：
Jones PP;Cebra JJ
用兔α链上存在的f和g基因座同种异型标记物的抗体进行荧光抗体染色，结果表明α链是派伊尔淋巴结淋巴细胞上的重链，之前已证明派伊尔淋巴结淋巴细胞是IgA产生浆细胞的前体。此外，发现用链霉蛋白酶剥离膜IG，然后培养过夜以允许内源性膜IG单独表达的淋巴细胞在其膜上具有μ链或α链，但不是两者都有。这些结果表明，大多数淋巴细胞仅限于合成一类重链的时间，并在淋巴细胞分化为浆细胞的过程中维持合成该特定重链的承诺。具有膜α链的淋巴细胞的比例在Peyer结和阑尾这两种肠相关淋巴组织（GALT）中高于其他淋巴组织。由于与非肠道相关组织相比，GALT是产生IgA的浆细胞前体的富集来源，因此携带膜α链的细胞的存在与这些组织产生伊加浆细胞的相对能力密切相关。
Fluorescent antibody staining with antibodies to the f and g locus allotype markers present on rabbit α-chains revealed that the α-chain is the heavy chain on the Peyer's patch lymphocytes which previously had been shown to be the precursors of IgA-producing plasma cells. In addition, lymphocytes which had been stripped of membrane Ig with pronase and then cultured overnight to allow the sole expression of endogenous membrane Ig were found to have either the µ-chain or the α-chain on their membranes, but not both. These results suggest that most lymphocytes are restricted to the synthesis of one class of heavy chains at a time and that the commitment to synthesizing that particular heavy chain is maintained during the differentiation of lymphocytes into plasma cells. The proportion of lymphocytes with membrane α-chains is higher in the Peyer's patch and appendix, two gut-associated lymphoid tissues (GALT), than in other lymphoid tissues. Since the GALT are enriched sources of precursors for IgA-producing plasma cells compared to nongut-associated tissues, the presence of cells bearing membrane α-chains correlates well with the relative abilities of these tissues to generate IgA plasma cells.