A quantitative framework for whole-body coordination reveals specific deficits in freely walking ataxic mice
A quantitative framework for whole-body coordination reveals specific deficits in freely walking ataxic mice
复制标题
全身协调的定量框架揭示了自由行走共济失调小鼠的特定缺陷
DOI：
10.7554/elife.07892
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发表时间：
2015-10-03
期刊：
影响因子：
7.7
通讯作者：
Carey, Megan R.
中科院分区：
文献类型：
--
作者：
Machado, Ana S.;Darmohray, Dana M.;Carey, Megan R.
全身运动的协调是运动控制的一个基本方面，但人们对其了解甚少。具有小脑回路缺陷的突变小鼠在运动协调方面表现出特征性的损伤；然而，这种步态共济失调的基本特征尚未被有效地分离出来。在这里，我们描述了一种新的系统（LocoMouse），用于分析自由行走小鼠的肢体、头部和尾部运动学。对明显的共济失调浦肯野细胞变性（pcd）小鼠的分析表明，虽然单个爪子向前运动的差异完全可以通过步行速度和身体大小的变化来解释，但更复杂的3D轨迹，特别是肢体间和全身协调受到损害。此外，pcd的协调缺陷与无法预测和补偿全身运动的后果是一致的。这些结果分离了小鼠全身协调的特异性损伤，并为理解小脑对协调运动的贡献提供了定量框架。DOI: 10.7554 / eLife.07892.001
The coordination of movement across the body is a fundamental, yet poorly understood aspect of motor control. Mutant mice with cerebellar circuit defects exhibit characteristic impairments in locomotor coordination; however, the fundamental features of this gait ataxia have not been effectively isolated. Here we describe a novel system (LocoMouse) for analyzing limb, head, and tail kinematics of freely walking mice. Analysis of visibly ataxic Purkinje cell degeneration (pcd) mice reveals that while differences in the forward motion of individual paws are fully accounted for by changes in walking speed and body size, more complex 3D trajectories and, especially, inter-limb and whole-body coordination are specifically impaired. Moreover, the coordination deficits in pcd are consistent with a failure to predict and compensate for the consequences of movement across the body. These results isolate specific impairments in whole-body coordination in mice and provide a quantitative framework for understanding cerebellar contributions to coordinated locomotion. DOI: 10.7554/eLife.07892.001