Interaction between chronic arsenic exposure via drinking water and plasma lactate dehydrogenase activity.
Interaction between chronic arsenic exposure via drinking water and plasma lactate dehydrogenase activity.
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DOI：
10.1016/j.scitotenv.2010.10.001
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发表时间：
2010-12
期刊：
影响因子：
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通讯作者：
Md. Rezaul Karim;K. Salam;Ekhtear Hossain;Khairul Islam;Nurshad Ali;A. Haque;Z. Saud;T. Yeasmin;M. Hossain;H. Miyataka;S. Himeno;Khaled Hossain
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文献类型：
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作者：
Md. Rezaul Karim;K. Salam;Ekhtear Hossain;Khairul Islam;Nurshad Ali;A. Haque;Z. Saud;T. Yeasmin;M. Hossain;H. Miyataka;S. Himeno;Khaled Hossain
砷是一种强有力的环境污染物，已造成人类文明史上最大的公共卫生中毒事件之一，影响到全世界数千万人，特别是在孟加拉国。血液中的乳酸脱氢酶（LDH）在预测细胞或器官损伤方面起着重要作用，并作为诊断各种癌症的重要线索。然而，慢性砷暴露对血液中LDH水平的影响尚未有文献报道。由于慢性砷暴露与器官损伤和多部位癌症有关，本研究旨在测定孟加拉国长期暴露于砷的人群的血浆LDH活性水平。在孟加拉国，共有185名生活在砷暴露地区的个人和121名生活在非暴露地区的个人被招募为研究对象。采用电感耦合等离子体质谱法（ICP-MS）测定饮用水、头发和指甲中砷含量，用分光光度计测定LDH活性。LDH活性随砷浓度的增加而显著增加。此外，根据每种暴露指标（水、头发和指甲砷浓度）的三种方式，将研究受试者分为四组，其中非暴露区域的研究受试者用作参考（最低暴露）组。LDH活性被发现在水和发砷浓度较高的暴露组中增加。LDH活性在低、中剂量砷暴露组也明显升高，提示血清LDH活性的升高可能有助于砷暴露者器官或组织损伤的早期预后。
Arsenic is a potent environmental pollutant that has caused one of the largest public health poisonings in the history of human civilization, affecting tens of millions of people worldwide especially in Bangladesh. Lactate dehydrogenase (LDH) in blood plays an important role in predicting cell or organ damage and as an important clue to the diagnosis of a variety of cancers. However, effect of chronic arsenic exposure on the LDH level in blood has not yet been documented. Since the chronic arsenic exposure is associated with organ damages and multi-site cancers, this research aimed at assaying the plasma level of LDH activity in the population who were exposed to arsenic chronically in Bangladesh. A total of 185 individuals living in arsenic-exposed areas and 121 individuals living in non-exposed area in Bangladesh were recruited as study subjects. Arsenic content in drinking water, hair and nails were estimated by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) and LDH activity was assayed by a spectrophotometer. Significant increase in LDH activity was observed with increasing concentrations of arsenic in water, hair and nails. Further, the study subjects were split into four groups based on the three ways of each exposure metrics (water, hair and nail arsenic concentrations) where the study subjects in the non-exposed area were used as a reference (lowest exposure) group. LDH activity was found to be increased in the higher exposure groups of water and hair arsenic concentrations. LDH activity was also increased at low to medium exposure groups of nail arsenic concentrations.Thus, the elevated plasma LDH activity might be helpful for the early prognosis of organ or tissue damage in the individuals who were exposed to arsenic chronically.