Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes
Dual-Color Imaging of Magnesium/Calcium Ion Activities with Two-Photon Fluorescent Probes
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DOI：
10.1021/ac302210v
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发表时间：
2012-10-02
影响因子：
7.4
通讯作者：
Cho, Bong Rae
中科院分区：
文献类型：
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作者：
Dong, Xiaohu;Han, Ji Hee;Cho, Bong Rae
我们报道的双光子探针(FMg1和FMg2)可以通过双光子显微镜选择性地检测活细胞和组织中的细胞内游离镁离子([Mg2+](I))。与BCAM结合，FMg2可以双色成像活细胞中的镁离子/钙离子活性和100-200微米深度的活组织中的[镁](I)/[钙](M)分布。
We report two-photon probes (FMg1 and FMg2) that can selectively detect intracellular free Mg2+ ([Mg2+](i)) in live cells and tissues by two-photon microscopy. Combined with BCaM, a two-photon probe for near-membrane Ca2+ ([Ca2+](m)), FMg2 allows dual-color imaging of Mg2+/Ca2+ activities in live cells and [Mg2+](i)/[Ca2+](m) distributions in live tissues at a depth of 100-200 mu m.