Recovery of methanotrophs from disturbance: population dynamics, evenness and functioning
Recovery of methanotrophs from disturbance: population dynamics, evenness and functioning
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DOI：
10.1038/ismej.2010.163
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发表时间：
2011-04-01
期刊：
影响因子：
11
通讯作者：
Frenzel, Peter
中科院分区：
文献类型：
--
作者：
Ho, Adrian;Lueke, Claudia;Frenzel, Peter
生物多样性被认为对生态系统的功能至关重要，但受到人为干扰的威胁。原核生物被认为在功能上是冗余的，实际上是不灭的。然而，最近的研究表明，微生物可能对环境干扰很敏感。以甲烷氧化菌为模式生物，通过将原生稻土与灭菌稻土以1:4和1:40的比例混合，模拟干扰引起的死亡，分别代表中度和重度死亡。将受干扰的微生物与未处理的对照组进行比较。通过甲烷吸收测量、pmoA-qPCR、基于pmoa的末端限制性片段长度多态性和基于pmoa的诊断芯片，在4个月的时间里恢复了活性和种群。在受干扰的微观环境中，甲烷氧化菌的多样性和均匀性下降，但功能没有受到损害。我们一致观察到在I型和II型甲烷化菌之间有明显的时间变化，并且与对照组相比，受干扰的微生物群体的快速增长导致了更高的细胞数量。过度补偿死亡率表明，对照组的种群规模受到与其他细菌竞争的限制。总的来说，甲烷氧化菌表现出了显著的补偿死亡的能力。ISME Journal (2011) 5,750 -758；doi: 10.1038 / ismej.2010.163;2010年10月28日在线发布
Biodiversity is claimed to be essential for ecosystem functioning, but is threatened by anthropogenic disturbances. Prokaryotes have been assumed to be functionally redundant and virtually inextinguishable. However, recent work indicates that microbes may well be sensitive to environmental disturbance. Focusing on methane-oxidizing bacteria as model organisms, we simulated disturbance-induced mortality by mixing native with sterilized paddy soil in two ratios, 1: 4 and 1: 40, representing moderate and severe die-offs. Disturbed microcosms were compared with an untreated control. Recovery of activity and populations was followed over 4 months by methane uptake measurements, pmoA-qPCR, pmoA-based terminal restriction fragment length polymorphism and a pmoA-based diagnostic microarray. Diversity and evenness of methanotrophs decreased in disturbed microcosms, but functioning was not compromised. We consistently observed distinctive temporal shifts between type I and type II methanotrophs, and a rapid population growth leading to even higher cell numbers comparing disturbed microcosms with the control. Overcompensating mortality suggested that population size in the control was limited by competition with other bacteria. Overall, methanotrophs showed a remarkable ability to compensate for die-offs. The ISME Journal (2011) 5, 750-758; doi:10.1038/ismej.2010.163; published online 28 October 2010