Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae.
Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae.
复制标题
DOI：
10.1083/jcb.86.1.123
复制
发表时间：
1980-07
影响因子：
7.8
通讯作者：
Schekman, R
中科院分区：
文献类型：
--
作者：
Field, C;Schekman, R
酿酒酵母分泌的细胞壁结合的酸性磷酸酶沿细胞表面的有限部分发生。在磷酸盐限制的生长介质上解压合成过程中产生的酸性磷酸酶活性用酶特异性染色检测，并最初定位于分裂细胞的芽部分。经过两到三代的磷酸盐限制生长，大多数细胞都可以染色；如果进一步的磷酸酶合成受到过量磷酸盐生长的抑制，就会产生分裂细胞，在这些细胞中，可以染色的是亲本细胞，而不是芽细胞。在α交配型细胞中，芽的生长被相反交配型细胞分泌的信息素(α因子)中断；细胞表面在α因子存在下继续生长，并产生特有的细胞尖端。当α因子处理启动酸性磷酸酶合成时，只有细胞尖可以染色；当α因子处理抑制磷酸合成时，细胞体可以染色，而细胞尖端不能染色。去抑制的α细胞和磷酸酶阴性的α细胞的混合物形成受精卵，在受精卵中主要有一个亲本细胞表面可以染色。细胞周期突变体CDC 24(Hartwell，L.H.1971.实验Cell Res.69：265-276)，不能发芽，而是在不允许的温度(37摄氏度)下对称地膨大为球形。在37℃的α因子处理过程中，该突变体不形成细胞尖端，虽然在此温度下会发生酸性磷脂分泌，但它不是定位的。这些结果表明，分泌物反映了酵母细胞表面生长的一种极性模式，这种组织需要CDC24基因产物。
Secretion of cell wall-bound acid phosphatase by Saccharomyces cerevisiae occurs along a restricted portion of the cell surface. Acid phosphatase activity produced during derepressed synthesis on a phosphate-limited growth medium is detected with an enzyme-specific stain and is localized initially to the bud portion of a dividing cell. After two to three generations of phosphate-limited growth, most of the cells can be stained; if further phosphatase synthesis is repressed by growth in excess phosphate, dividing cells are produced in which the parent but not the bud can be stained. Budding growth is interrupted in α-mating-type cells by a pheromone (α-factor) secreted by the opposite mating type; cell surface growth continues in the presence of α-factor and produces a characteristic cell tip. When acid phosphatase synthesis is initiated during α-factor treatment, only the cell tip can br stained; when phosphate synthesis is repressed during α-factor treatment, the cell body but not the tip can be stained. A mixture of derepressed α cells and phosphatase-negative α cells form zygotes in which mainly one parent cell surface can be stained. The cell cycle mutant, cdc 24 (Hartwell, L.H. 1971. Exp. Cell Res. 69:265-276), fails to bud and, instead, expands symmetrically as a sphere at a nonpermissive temperature (37 degrees C). This mutant does not form a cell tip during α-factor treatment at 37 degrees C, and although acid phosphatade secretion occurs at this temperature, it is not localized. These results suggest that secretion reflects a polar mode of yeast cell- surface growth, and that this organization requires the cdc 24 gene product.