Light intensity impacts on growth, molting and oxidative stress of juvenile mud crab Scylla paramamosain
Light intensity impacts on growth, molting and oxidative stress of juvenile mud crab Scylla paramamosain
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光强对拟青蟹幼蟹生长、蜕皮和氧化应激的影响
DOI：
10.1016/j.aquaculture.2021.737159
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发表时间：
2021-07-14
期刊：
影响因子：
4.5
通讯作者：
Mu, Changkao
中科院分区：
文献类型：
--
作者：
Chen, Shujian;Migaud, Herve;Mu, Changkao
通过8周的回归分析，研究了光照强度从黑暗增加到30 W m- 2对拟穴青蟹生长、蜕皮、抗氧化能力以及蜕皮和蜕皮相关基因表达的影响。两种处理之间的存活率无显著差异（实验结束时范围为71.7%至87.3%）。然而，体重增加和特定的增长率在研究期间显示曲线响应光处理的峰值在螃蟹暴露于10 W m- 2。线性（BLM），二次（BQM）和4-参数饱和动力学（4-SKM）模型表明，SGR的最佳光照强度分别为12.98，18.27和11.36 W m- 2。光照强度似乎没有影响蜕皮。然而，在黑暗中饲养的螃蟹显示出显着降低蜕皮频率（3.51 +/- 0.16）和延长蜕皮间隔相比，其他治疗。在眼柄褪黑激素水平显着较高的螃蟹暴露于黑暗（502.52 +/- 56.24 pg mL-1）比光强度为10-30 W m- 2（413.50 +/- 32.38和384.99 +/- 15.56 pg mL-1）。皮质醇水平显着降低0和5 W m- 2组。光照强度显著影响抗氧化酶的活性，在10 W m- 2光照下，螃蟹的总抗氧化能力（T-AOC）显著增加，在15 W m- 2光照下，螃蟹的过氧化氢酶（CAT）和超氧化物歧化酶（SOD）显著增加，丙二醛（MDA）显著降低。蜕皮抑制激素（MIH）的基因表达下调眼柄蟹暴露于10 W m- 2相比，黑暗和20-30 W m- 2。光合作用相关基因的表达没有表现出明显的光强变化趋势。综上所述，这些结果表明光强度可以影响S。拟穴龙的生长、蜕皮、应激水平和抗氧化能力。因此，应仔细考虑螃蟹养殖中使用的光照制度，以优化生产力和福利。
An 8 weeks regression study was performed to test the effects of increasing light intensities from darkness to 30 W m- 2 on growth performance, molting, antioxidant capacity, and gene expression of molting and apoptosisrelated genes in Scylla paramamosain. No significant differences were found in survival rates between treatments (ranging from 71.7 to 87.3% at the end of the experiment). However, weight gain and specific growth rate over the study period displayed a curvilinear response to light treatments with peak values in crabs exposed to 10 W m- 2. Linear (BLM), quadratic (BQM), and 4-parameter saturation kinetic (4-SKM) models show the optimal light intensities for SGR were 12.98, 18.27, and 11.36 W m- 2, respectively. The light intensity did not appear to impact molting. However, crabs reared in darkness showed significantly reduced molt frequency (3.51 +/- 0.16) and extended intermolt intervals compared to other treatments. Melatonin levels in the eyestalks were significantly higher in crabs exposed to darkness (502.52 +/- 56.24 pg mL-1) than light intensities of 10-30 W m- 2 (413.50 +/- 32.38 and 384.99 +/- 15.56 pg mL-1). Cortisol levels were significantly lower in the 0 and 5 W m- 2 groups. Light intensity significantly impacted the activity of antioxidant enzymes, with crabs showing a significant increase in total antioxidant capacity (T-AOC) under 10 W m- 2, catalase (CAT), and superoxide dismutase (SOD) under 15 W m- 2 and lower malondialdehyde (MDA). Gene expression of the molt-inhibiting hormone (MIH) was downregulated in eyestalks from crabs exposed to 10 W m- 2 compared to darkness and 20-30 W m- 2. Expression of apoptosis-related genes did not show clear light intensity trends. Taken together, these results suggest light intensity can impact S. paramamosain growth, molting, stress levels, and antioxidant capacity. As such, light regimes used in crab farming should be carefully considered to optimize productivity and welfare.