Epididymal sperm transport in normal and recent spinal cord injured Sprague Dawley rats.
Epididymal sperm transport in normal and recent spinal cord injured Sprague Dawley rats.
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正常和最近脊髓损伤的斯普拉格道利大鼠的附睾精子运输。
DOI：
10.1080/10790268.1999.11719554
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Huang,HF
中科院分区：
文献类型：
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作者：
Linsenmeyer,TA;Ottenweller,JE;D'Imperio,JS;Pogach,LM;Huang,HF
脊髓损伤（SCI）后精液质量差的原因尚不清楚。一个可能的原因是，根据研究报告，在几次人工射精后，SCI男性的精液质量有所改善，这是由于附睾精子运输延迟。我们的研究旨在建立Sprague Dawley大鼠附睾精子运输的基线值，并评估脊髓损伤对这一过程的影响。用氚化精氨酸标记精蛋白，注射后多次处死大鼠。每个附睾从近端到远端分成六个相等的部分。精子尾部用8摩尔(M)尿素在2mM二硫苏糖醇（DTT）存在下溶解；离心（3000转/分钟，10分钟）收集精子头。每个切片的精子头中的放射性被计数，并以每百万精子头的计数表示。为了考虑不同的标记精氨酸掺入率，计算每个切片中每百万精子头的计数百分比，相对于所有六个切片的计数总数。我们的结果显示精子有序地通过附睾。非脊髓损伤大鼠标记精子进入附睾需要8天，在尾（尾）段需要28天达到峰值。与假对照组的转运相比，大鼠在T-9脊髓损伤后10天出现停滞。T-9横切动物附睾近端（1、2、4）标记精子的百分比显著增加（p < 0.01）证明了这一点。如果SCI男性出现类似的瘀滞，显然会导致精液质量差。然而，这种停滞状态在大鼠脊髓损伤后持续多久仍有待确定。（中华脊柱杂志1999；22:102-106）
Causes of poor semen quality following spinal cord injury (SCI) are not known. One possible reason, based upon studies that reported improved semen quality in SCI men after several induced ejaculations, is delayed epididymal sperm transport. Our study was designed to establish baseline epididymal sperm transport values in the Sprague Dawley rat and evaluate effects of SCI on this process. Spermatozoa protamine was labeled with tritiated arginine, and the rats were sacrificed various times after injection. Each epididymis was divided into six equal sections from proximal to distal. Sperm tails were dissolved with 8 molar (M) urea in the presence of 2mM dithiothreitol (DTT); sperm heads were collected by centrifugation (3,000 rpms, 10 min.). The radioactivity in sperm heads from each section was counted and expressed as counts per million sperm heads. To account for different rates of labeled arginine incorporation, the percentage of counts per million sperm heads in each section was calculated relative to the total number of counts in all six sections. Our results showed there was an orderly progression of sperm through the epididymis. It took 8 days for labeled sperm to enter the epididymis and 28 days to peak in the caudal (tail) section in non-SCI rats. Stasis was present 10 days after T-9 SCI in rats compared with transport in sham controls. This was evidenced by a significant increase in the percentage of labeled sperm in proximal sections of the epididymis (sections 1, 2, and 4) in T-9 transected animals (p < 0.01). If similar stasis occurs in SCI men, it could obviously contribute to poor semen quality. However, it remains to be determined how long this stasis persists after SCI in rats.(J Spinal Corel Med1999;22:102–106)