Interaction of myotoxin a with artificial membranes: Raman spectroscopic investigation.
Interaction of myotoxin a with artificial membranes: Raman spectroscopic investigation.
复制标题
肌毒素 a 与人工膜的相互作用：拉曼光谱研究。
作者：
Liddle,WK;Tu,AT
来自 Crotalus viridis viridis（草原响尾蛇）毒液的肌毒素 a 是一种小蛋白质，可导致肌肉坏死。它是一种碱性蛋白质，具有已知序列的 42 个氨基酸残基。三个二硫键赋予其高度紧凑的结构。对毒素作用的显微镜检查表明，最明显和最早可见的损伤发生在细胞内，即骨骼肌的肌浆网膜系统中。因此，更好地了解其作用机制特别有意义。使用二肉豆蔻酰磷脂酰胆碱 (DMPC) 和二肉豆蔻酰磷脂酰丝氨酸 (DMPS) 研究了肌毒素 a 与人工膜（多层磷脂分散体）的相互作用。检查拉曼光谱的两个区域以获取信息：2800 至 3000 cm" 1 之间的 CH 拉伸区域和 1000 至 1300 cm" 1 之间的 CC 拉伸区域。确定了肌毒素 a 对人造膜热致相行为的影响。这是通过监测三个结构敏感的拉曼强度比 72932/2880 来完成的，
Myotoxin a from the venom of Crotalus viridis viridis (prairierattlesnake) is a small protein which is responsible for myonecrosis. It is a basic protein with 42 amino acid residues of known sequence. Three disulfide bonds give it a highly compact structure. Microscopic examination of the toxin’s effects reveals that themost pronounced and earliest visible damage occurs intracellularly, in the sarcoplasmic reticulum membrane system of skeletal muscle. A better understanding of its mechanism of action is therefore of particular interest. The interaction of myotoxin a with artificial membranes (multibilamellar phospholipid dispersions) was investigated by using dimyristoylphosphatidylcholine (DMPC) and dimyristoyl-phosphatidylserine (DMPS). Two regions of the Raman spectrum were examined for information: the CH stretching region between 2800 and 3000cm" 1 and the CC stretching region between 1000 and 1300 cm" 1. The effects of myotoxin a on the thermotropic phase behavior of the artificial membranes were determined. This was done by monitoring three structurally sensitive Raman intensity ratios, 72932/2880,