Commentary on Tam and Zhou, 1996.
Commentary on Tam and Zhou, 1996.
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Tam 和 Zhou 的评论，1996。
DOI：
10.1016/j.ydbio.2019.02.006
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发表时间：
2019
影响因子：
2.7
通讯作者：
Soriano,Philippe
中科院分区：
文献类型：
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作者：
Soriano,Philippe
在这篇对《发育生物学》多年来发表的经典论文的评论中，我选择了Tam和Zhou（1996）关于外胚层细胞（EPI）在小鼠胚胎生殖细胞谱系中的可塑性贡献的论文。我在我的发育生物学课上教这篇论文，学生们都很喜欢。一段时间以来，人们已经知道单个EPI细胞从一个着床前胚胎移植到另一个胚胎中可以产生原始生殖细胞（PGCs）和体细胞组织（Gardner, 1968）。然而，植入子宫后，使用染料注射的英雄命运定位实验表明，只有邻近胚胎外外胚层（ExE）的EPI细胞才能产生PGCs，这提高了这些细胞可能被胚胎外信号诱导的可能性（Lawson和Hage， 1994）。在这篇重要的论文中，Tam和Zhou（1996）通过在原肠胚中进行技术上具有挑战性的移植实验，最终解决了这个问题。他们发现，远离ExE的远端EPI细胞在转移到近端位置时可以形成PGCs。相反，近端EPI细胞移植到远端通常不能形成生殖细胞。这些实验表明EPI细胞在细胞命运方面具有可塑性，并依赖于它们在胚胎中的定位来形成PGCs。反过来，这强烈表明PGCs是在这个阶段通过响应ExE信号的诱导过程形成的。这篇论文非常有影响力，对该领域产生了显著的影响，因为它为寻找这样一种诱导剂奠定了基础，随后被证明是BMP信号传导（Lawson et al., 1999）。
For this commentary on classic papers published by Developmental Biology over the years, I chose to highlight this manuscript by Tam and Zhou (1996) on the plasticity of epiblast (EPI) cell contribution to the germ cell lineage in the mouse embryo. I teach this paper in my Developmental Biology class and students always love it.It had been known for some time that single EPI cells transplanted from one preimplantation embryo to another could give rise to both primordial germ cells (PGCs) and somatic tissues (Gardner, 1968). Following implantation into the uterus however, heroic fate mapping experiments using dye injections indicated that only EPI cells neighboring the extraembryonic ectoderm (ExE) could give rise to PGCs, raising the possibility that these cells might be induced by an extraembryonic signal (Lawson and Hage, 1994). In this important paper, Tam and Zhou (1996) addressed the issue conclusively, by performing technically challenging transplantation experiments in gastrulating embryos. They found that distal EPI cells, far removed from the ExE, could form PGCs when transferred to a proximal location. Conversely, proximal EPI cells transplanted to a distal site generally did not form germ cells. These experiments indicated that EPI cells are plastic with respect to cell fate and depend on their localization in the embryo to form PGCs. This, in turn, strongly suggested that PGCs are formed at this stage through an induction process in response to a signal from the ExE. The paper was quite influential and had a marked impact on the field, as it set the stage for the search for such an inducer, subsequently shown to be BMP signaling (Lawson et al., 1999).