Simultaneous liquid chromatography tandem mass spectrometric determination of urinary free metanephrines and catecholamines, with comparisons of free and deconjugated metabolites
Simultaneous liquid chromatography tandem mass spectrometric determination of urinary free metanephrines and catecholamines, with comparisons of free and deconjugated metabolites
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DOI：
10.1016/j.cca.2012.12.031
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发表时间：
2013-03-15
影响因子：
5
通讯作者：
Eisenhofer, Graeme
中科院分区：
文献类型：
--
作者：
Peitzsch, Mirko;Pelzel, Daniela;Eisenhofer, Graeme
目的：介绍一种新的液相色谱串联质谱同时测定尿儿茶酚胺及其游离o -甲基化代谢物的方法，并将其与去甲基化代谢物进行比较。方法：对方法的回收率、线性度、精密度和准确度、分析物稳定性、离子抑制和携带性进行验证。对53例嗜铬细胞瘤患者和138例非嗜铬细胞瘤患者的结果进行了比较。结果：分析物回收率为60% ~ 96%，测定内、测定间变异系数为2.7 ~ 13.2%。该方法具有3个数量级以上的良好线性，分析灵敏度足以测量至1.2 nmol/L的游离o -甲基化代谢物，排泄率低于去共轭代谢物的20%，但易于测量。嗜铬细胞瘤患者尿中去甲肾上腺素的增加相对于志愿者，游离的高于去钠化代谢物，两者都高于去甲肾上腺素（10倍vs 5.5倍vs 3.7倍）。相比之下，尿中游离肾上腺素（2.7和3.2）和甲氧基酪胺（2.1和1.9）的相对增加没有差异，但甲氧基酪胺的相对增加大于多巴胺（12）。结论：用本方法检测尿儿茶酚胺及其游离o -甲基化代谢物在诊断嗜铬细胞病方面具有潜在的优势。(C) 2013 Elsevier B.V.版权所有
Objective: We introduce a novel liquid chromatographic tandem-mass spectrometric method for simultaneous measurements of urinary catecholamines and their free O-methylated metabolites, which we compare to the deconjugated metabolites.Methods: Method performance was validated for recovery, linearity, precision and accuracy, analyte stability, ion suppression and carry over. Results from 53 patients with and 138 volunteers without pheochromocytoma were compared.Results: Analyte recoveries ranged from 60 to 96% and intra- and inter-assay coefficients of variation from 2.7 to 13.2%. The method showed excellent linearity over 3 orders of magnitude with analytical sensitivity sufficient to measure to 1.2 nmol/L Free O-methylated metabolites were excreted at less than 20% the rates of the deconjugated metabolites, but were easily measureable. Increases in urinary normetanephrine in pheochromocytoma patients relative to volunteers were higher for free than deconjugated metabolites and higher for both than for norepinephrine (10 vs 5.5 vs 3.7 fold increases). In contrast, relative increases in urinary free versus deconjugated metanephrine (2.7 and 3.2) and methoxytyramine (2.1 and 1.9) did not differ, but for methoxytyramine were larger than for dopamine (12).Conclusion: Measurements of urinary catecholamines and their free O-methylated metabolites by our method provide potential advantages over urinary deconjugated metanephrines for diagnosis of pheochromocytorna. (C) 2013 Elsevier B.V. All rights reserved.